Izvestiya of Saratov University.

Physics

ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)

For citation:

Zyuryukina O. A., Sinichkin Y. P. Dehydration of Biotissues During Their Compression. Izvestiya of Saratov University. Physics , 2020, vol. 20, iss. 2, pp. 92-102. DOI: 10.18500/1817-3020-2020-20-2-92-102

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Published online: 
01.06.2020
Full text:
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(downloads: 417)
Language: 
Russian
Heading: 
Biophysics and medical physics
UDC: 
535.2:535.8
DOI: 
10.18500/1817-3020-2020-20-2-92-102

Dehydration of Biotissues During Their Compression

Autors: 
Zyuryukina Olga Anatolievna, Saratov State University
Sinichkin Yury Petrovich, Saratov State University
Abstract: 

Background and Objectives: Dehydration of tissue is one of the possible mechanisms of mechanical tissue optical clearing. In this study we investigated the effects of dehydration of ex vivo cow muscle tissue samples during their compression on diffuse reflectance spectra of the tissue. The purpose of research was to identify the correlation between the diffuse reflectance of the tissue and its dehydration. Materials and Methods: The dehydration of tissue samples of 20×20×20 mm^3 in size was carried out by drying the samples for 17 hours and the dehydration of cylindrical tissue samples with diameter 20 mm and thickness of 25 mm was carried by compressing the samples in a specially designed cell with a nozzle on a fiber optic sensor. In the first case the samples were weighed every hour and the diffuse reflectance spectrum was recorded. In the second case the compression value changed every 6 minutes, before which the samples were weighed and the diffuse reflectance spectrum was also recorded. Results: The dynamics of the reduced weight of the samples both in the dying process and in the compression process were determined. At the end of the drying process, the weights of the samples decreased by 70%. This value corresponded to the degree of hydration of the samples before the experiments. When the pressure on the sample was about 110 kPa, the weights of the samples decreased by 45%. The maximum degree of dehydration of the samples during compression was about 50%. The dehydration of the samples during their drying was accompanied by an increase in their diffuse reflectance. On the contrary, the application of compression led to a decrease in the diffuse reflectance according to a two-exponential law, which is may be explained by the difference in the physical structures of the dehydrated samples. Conclusion: The processes of reducing the diffuse reflectance of the sample and its weight during compression correlate well. As a result, the analysis of changes in the diffuse reflectance of the tissue sample as a result of its compression allows us to evaluate changes in the hydration of the sample. The choice of the wavelength of the probe light can give not only a qualitative, but also quantitative agreement between the two processes. \

Key words: 
muscul tissue
dehydration
compression
diffuse reflection
optical clearing
Reference: 
  1. Tuchin V. V. Optical clearing of tissues and blood. Bellingham, WA, USA, SPIE Press, 2005, vol. PM 154. 254 p.
  2. Rylander C. G., Stumpp O. F., Milner T. E., Kemp N. J., Mendenhall J. M., Diller K. R., Welch A. J. Dehydration mechanism of optical clearing in tissue. J. Biomed. Opt., 2006, vol. 11, no. 4, pp. 041117.
  3. Chan E. K., Sorg B., Protsenko D., O’Neil M., Motamedi M., Welch A. J. Effects of compression on soft tissue optical properties. IEEE J. Select. Top. Quant. Electron., 1996, vol. 2, no. 4, pp. 943–950.
  4. Sinichkin Yu. P., Utz S. R., Pilipenko H. A. Spectroscopy of the human sin in vivo: 1. Refl ectance spectra. Opt. and Spect., 1996, vol. 80, no. 2, pp. 260–267.
  5. Shangguan H., Prahl S. A., Jacques S. L., Casperson L. W., Gregory K.W. Pressure effects on soft tissues monitored by changes in tissue optical properties. Proc. SPIE., 1998, vol. 3254, pp. 366–371.
  6. Chen W., Liu R., Xu K., Wang R. K Infl uence of contact state on NIR diffuse refl ectance spectroscopy in vivo. J. Phys. D, 2005, vol. 38, pp. 26913695.
  7. Reif R., Amorosino M. S., Calabro K. W., A’Amar O., Singh K. S., Bigio I. J. Analysis of change in refl ectance measurements on biological tissues subjected to different probe pressures. J. Biomed. Opt., 2008, vol. 13, no. 1, pp. 010502.
  8. Ti Y., Lin W.C. Effects of probe contact pressure on in vivo optical spectroscopy. Opt. Express, 2008, vol. 16, no. 6, pp. 4250–4262.
  9. Rylander C. G., Milner T. E., Baranov S. A., Nelson J. S. Mechanical tissue optical clearing devices: Enhancement of light penetration in ex vivo porcine skin and adipose tissue. Laser Surg. Med., 2008, vol. 40, no. 10, pp. 688–694.
  10. Delgado Atencio J. A., Orozco Guillen E. E., Vazquezy Montiel S., Cunill Rodríguez M., Castro Ramos J., Gutiérrez J. L., Martínez F. Infl uence of probe pressure on human skin diffuse refl ectance spectroscopy measurements. Opt. Mem. Neur. Networks (Information Optics), 2009, vol. 18, no. 1, pp. 6–14.
  11. Vogt W. C., Shen H., Wang G., Rylander C. G. Parametric study of tissue optical clearing by localized mechanical compression using combined fi nite element and Monte Carlo simulation. J. Innov. Opt. Health Sci., 2010, vol. 3, no. 3, pp. 203–211.
  12. Izquierdo Roman A., Vogt W. C., Hyacinth L., Rylander C. G. Mechanical tissue optical clearing technique increases imaging resolution and contrast through ex vivo porcine skin. Laser Surg. Med., 2011, vol. 43, pp. 814–823.
  13. Gurjarpadhye A., Vogt W. C., Liu Y., Rylander C. G. Effect of localized mechanical indentation on skin water content evaluated using OCT. Int. J. Biomed. Imaging, 2011, vol. 2011, pp. 817250.
  14. Lim L. A., Nichols B., Rajaram N., Tunnell J. W. Probe pressure effects on human skin diffuse refl ectance and fl uorescence spectroscopy measurements. J. Biomed. Opt., 2011, vol. 16, no. 1, pp. 011012.
  15. Vogt W. C., Izquierdo-Roman A., Nichols B., Lim L., Tunnell J. W., Rylander C. G. Effects of mechanical indentation on diffuse refl ectance spectra, light transmission, and intrinsic optical properties in ex vivo porcine skin. Laser Surg. Med., 2012, vol. 44, pp. 303–309.
  16. Cugmas B., Bürmena M., Bregar V., Pernuša F., Likar B. Pressure-induced near infrared spectra response as a valuable source of information for soft tissue classifi cation. J. Biomed. Opt., 2013, vol. 18, no. 4, pp. 047002.
  17. Li C., Jiang J., Xu K. The variations of water in human tissue under certain compression: studied with diffuse refl ectance spectroscopy. Journal of Innovative Optical Health Sciences, 2013, vol. 6, no. 1, pp. 1350005.
  18. Nakhaeva I. A., Mohammed R. M., Zyuryukina O. A., Sinichkin Yu. P. Effect of external mechanical pressure on optical properties of the human skin in vivo. Opt. and Spectrosc., 2014, vol. 117, no. 3, pp. 506–512.
  19. Nakhaeva I. A., Zyuryukina O. A., Mohammed R. M., Sinichkin Yu. P. The effect of external mechanical compression on in vivo water content in human skin. Opt. and Spectr., 2015, vol. 118, no. 5, pp. 834–840.
  20. Zyuryukina O. A., Sinichkin Yu. P. The dynamics of optical and physiological characteristics of human skin in vivo during its compression. Opt. and Spectr., 2019, vol. 127, no. 3, pp. 555–561.
  21. Xu X., Wang R. K. The role of water desorption on optical clearing of biotissue: Studied with near infrared refl ectance spectroscopy. Med. Phys., 2003, vol. 30, no. 6, pp. 1246–1253.
  22. Xu X., Wang R. K. Synergistic effect of hyperosmotic agents of dimethyl sulfoxide and glycerol on optical clearing of gastric tissue studied with near infrared spectroscopy. Phys. Med. Biol., 2004, vol. 49, pp. 457–468.
  23. Hardisty M. R., Kienle D. F., Kuhl T. L., Stover S. M., Fyhrie D. P. Strain-induced optical changes in demineralized bone. Journal of Biomedical Optics, 2014, vol. 19, no. 3, pp. 035001.
  24. Genina E. A., Bashkatov A. N., Korobko A. A., Zubkova E. A., Tuchin V. V., Yaroslavsky I., Altshuler G. B. Optical clearing of human skin: comparative study of permeability and dehydration of intact and photothermally perforated skin. J. Biomed. Opt., 2008, vol. 13, no. 2, pp. 021102.
  25. Dolotov L. E., Sinichkin Yu. P. Features of fi ber optic probes applications in tissues spectral measurements. Optics and Spectroscopy, 2013, vol. 115, no. 2, pp. 40–46.
  26. Schmitt J. M., Kumar G. Optical scattering properties of soft tissue: a discrete particle model. Appl. Opt., 1998, vol. 37, no. 13, pp. 2788–2797.
  27. Shvachkina M. E., Yakovlev D. D., Lazareva E. N., Pravlin A. B., Yakovlev D. A. Monitoring of the process of immersion optical clearing of collagen bundles using optical coherence tomography. Opt. and Spectr., 2019, vol. 127, no. 2, pp. 359–367.
  28. Farrell T. J., Patterson M. S., Wilson B. A diffuse theory model of spatially resolved, steady-state diffuse refl ectance for the noninvasive determination of tissue optical properties in vivo. Med. Phys., 1992, vol. 19, pp. 879–888.
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