Izvestiya of Saratov University.

Physics

ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)

For citation:

Ерохин П. С., Utkin D. V., Кузнецов О. С., Konnov N. P., Aspen N. A. Application of Atomic Force Microscopy for Detection of Influence of Antibiotic Upon the Microbial Cell (on the Model of E.coli and I Generation Cephalosporins). Izvestiya of Saratov University. Physics , 2013, vol. 13, iss. 2, pp. 28-33. DOI: 10.18500/1817-3020-2013-13-2-28-33

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Full text:
download
(downloads: 165)
Language: 
Russian
Heading: 
Physics
UDC: 
579.23:53.086:615.281
DOI: 
10.18500/1817-3020-2013-13-2-28-33

Application of Atomic Force Microscopy for Detection of Influence of Antibiotic Upon the Microbial Cell (on the Model of E.coli and I Generation Cephalosporins)

Autors: 
Ерохин Павел Сергеевич, Russian Research Anti-Plague Institute «Microbe»
Utkin Denis Valer'evich, Russian Research Anti-Plague Institute «Microbe»
Кузнецов Олег Святославович, Russian Research Anti-Plague Institute «Microbe»
Konnov Nicholas Pavlovich, Russian Research Anti-Plague Institute «Microbe»
Aspen Natalia Aleksandrovna, Russian Research Anti-Plague Institute «Microbe»
Abstract: 

Alteration of E.coli cell wall caused by Cefazolin-AKOS was observed atomic force microscopy (AFM). Using semi-contact and contact modes the damaging effect of the Cefazolin-AKOS antibiotic was shown after a 30 minutes exposure. The assessment of physical parameters of cell allow to get more detailed information on the effect of antibiotics on microorganisms.

Key words: 
AFM
semi-contact mode
contact mode
microorganisms
antibacterial agents
roughness
adhesion force
distribution lateral characteristics
sensitivity
Reference: 
  1. МУК 4.2.1890-04. Определение чувствительности микроорганизмов к антибактериальным препаратам. Методические указания. М., 2004. 91 с.
  2. Saranya S., Hemashenpagam N. Antagonistic activity and antibiotic sensitivity of Lactic acid bacteria from fermented dairy products // Adv. in Appl. Sci. Res. 2011. Vol. 2, № 4. P. 528–534.
  3. Liasi S. A., Azmi T. I., Hassan M. D., Shuhaimi M., Rosfarizan M., Ariff A. B. Antimicrobial activity and antibiotic sensitivity of three isolates of lactic acid bacteria from fermented fi sh product, Budu // Malaysian J. of Microbiol. 2009. Vol. 5, № 1. P. 33–37.
  4. Fuhrmann A., Ros R. Single-molecule force spectroscopy : a method for quantitative analysis of ligandreceptor interactions // Nanomedicine. 2010. Vol. 5, № 4. P. 657–666.
  5. Deisingh A. Biosensors for microbial detection // Microbiologist. 2003. Vol. 2. P. 30–33.
  6. Lourenco F. R., Pinto T. de J. A. Antibiotic microbial assay using kinetic-reading microplate system // Braz. J. of Pharm. Sci. 2011. Vol. 47, № 3. P. 573–584.
  7. Vora G. J., Meador C. E., Bird M. M., Bopp C. A., Andreadis J. D., Stenger D. A. Microarray-based detection of genetic geterogeneity, antimicrobial resistance and the viable but nonculturable state in human pathogenic Vibrio spp. // PNAS. 2005. Vol. 102, № 52. P. 19109– 19114.
  8. Braga P.C., Ricci D. Atomic force microscopy : application to investigation of Escherichia coli morphology before and after exposure to cefodizime // Antimicrob. agents and chemother. 1998. Vol. 42, № 1. P. 18–22.
  9. Nikiyan H., Vasilchenko A., Deryabin D. AFM investigations of various disturbing factors on bacterial cells // Formatex. 2010. P. 523–529.
  10. Ouberai M., Garch F. E., Bussiere A., Riou M., Alsteens D., Lins L., Baussanne I., Dufrene Y. F., Brasseur R., Decout J.-L., Mingeot-Leclercq M.-P. The Pseudomonas aeruginosa membranes: A target for a new amphiphilic aminoglycoside derivative? // Biochim. et Biophys. Acta. 2011. Vol. 1808. P. 1716–1727.
  • 1065 reads