Izvestiya of Saratov University.
ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)


микроорганизмы

A Complex Approach for the Study of Biofilms of Microorganisms by Atomic Force Microscopy

The biofilms of Staphylococcus aureus and Escherichia coli have been investigated by atomic force microscopy (AFM). The usage of complex approach of semi-contact AFM mode has shown that the S. aureus and E. coli biofilms represent an organized community of microorganisms. Semicontact error mode and phase imaging mode investigation of extracellular matrix of S. aureus and E. coli biofilms, confirms dimensions of bacteria and biofilms. Semicontact mode let a development S-layer on surface of microorganisms, contact – different in adhesive force of bacteria.

Current Abilities Investigation Ultrastucture Cells of Microorganisms Using Method Scanning Probe Microscopy

This review presents data about current application of scanning probe microscopy in microbiology research. Methods of scanning probe microscopy belong used to study the ultrastructural and morphological features of microorganisms, their mechanical properties et al. The review also present data about the development methods of scanning probe microscopy to study microorganisms and their communities.

Application of Atomic Force Microscopy for Detection of Influence of Antibiotic Upon the Microbial Cell (on the Model of E.coli and I Generation Cephalosporins)

Alteration of E.coli cell wall caused by Cefazolin-AKOS was observed atomic force microscopy (AFM). Using semi-contact and contact modes the damaging effect of the Cefazolin-AKOS antibiotic was shown after a 30 minutes exposure. The assessment of physical parameters of cell allow to get more detailed information on the effect of antibiotics on microorganisms.