Izvestiya of Saratov University.

Physics

ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)

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Ерохин П. С., Кузнецов О. С., Konnov N. P., Vidyaeva N. A., Utkin D. V. A Complex Approach for the Study of Biofilms of Microorganisms by Atomic Force Microscopy. Izvestiya of Saratov University. Physics , 2012, vol. 12, iss. 1, pp. 42-46. DOI: 10.18500/1817-3020-2012-12-1-42-46

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
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Language: 
Russian
Heading: 
Physics
UDC: 
579.23:53.086:615.281
DOI: 
10.18500/1817-3020-2012-12-1-42-46

A Complex Approach for the Study of Biofilms of Microorganisms by Atomic Force Microscopy

Autors: 
Ерохин Павел Сергеевич, Russian Research Anti-Plague Institute «Microbe»
Кузнецов Олег Святославович, Russian Research Anti-Plague Institute «Microbe»
Konnov Nicholas Pavlovich, Russian Research Anti-Plague Institute «Microbe»
Vidyaeva Nadezhda Andreevna, Russian Research Anti-Plague Institute «Microbe»
Utkin Denis Valer'evich, Russian Research Anti-Plague Institute «Microbe»
Abstract: 

The biofilms of Staphylococcus aureus and Escherichia coli have been investigated by atomic force microscopy (AFM). The usage of complex approach of semi-contact AFM mode has shown that the S. aureus and E. coli biofilms represent an organized community of microorganisms. Semicontact error mode and phase imaging mode investigation of extracellular matrix of S. aureus and E. coli biofilms, confirms dimensions of bacteria and biofilms. Semicontact mode let a development S-layer on surface of microorganisms, contact – different in adhesive force of bacteria.

Key words: 
atomic force microscopy
extracellular matrix
biofilm
microorganisms
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