Izvestiya of Saratov University.


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ISSN 2542-193X (Online)

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Genina E. A., Bashkatov A. N., Tuchin V. V. Study of Ethanol Impact on the Transepidermal Transport of Indocyanine Green with Backscattering Spectroscopy. Izvestiya of Saratov University. Physics , 2016, vol. 16, iss. 2, pp. 91-96. DOI: 10.18500/1817-3020-2016-16-2-91-96

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Study of Ethanol Impact on the Transepidermal Transport of Indocyanine Green with Backscattering Spectroscopy

Genina Elina Alekseevna, Saratov State University
Bashkatov Alexey Nikolaevich, Saratov State University
Tuchin Valeriy Viktorovich, Saratov State University

In the work, impact of aqueous 40%-ethanol solution on the transepidermal transport of photodynamic dye indocyanine green has been studied with backscattering spectroscopy. It has been first obtained the value of the dye diffusion coefficient through the stratum corneum ex vivo at the use of ethanol as a diffusion enhancer: (6.85±3.75)×10−7 cm2/s.

  1. Blank I. H., Moloney J., Emslie A. G., Simon I., Apt C. The diffusion of water across the stratum corneum as a function of its water content // J. Invest. Dermatol. 1984. Vol. 82. P. 188‒194.
  2. Sennhenn B., Giese K., Plamann K., Harendt N., Kolmel K. In vivo evaluation of the penetration of topically applied drugs into human skin by spectroscopic method // Skin Pharmacol. 1993. Vol. 6. P. 152‒160.
  3. Mitragotri S. In situ determination of partition and diffusion coeffi cients in the lipid bilayers of stratum corneum // Pharm. Res. 2000. Vol. 17. P. 1026‒1029.
  4. Weigmann H.-J., Lademann J., Schanzer S., Lindemann U., von Pelchrzim R., Schaefer H., Sterry W., Shah V. Correlation of the local distribution of topically applied substances inside the stratum corneum determined by tape stripping to differences in bioavai lability // Skin Pharmacol. Appl. Skin Physiol. 2001. Vol. 14. P. 93‒103.
  5. Star W. M., Aalders M. C. G., Sac A., Sterenborg H.J.C.M. Quantitative model calculation of the time-dependent Protoporphyrin IX concentration in normal human epidermis after delivery of ALA by passive topical application or iontophoresis // Photochem. Photobiol. 2002. Vol. 75, № 4. P. 424-432.
  6. Genina E. A., Bashkatov A. N., Korobko A. A., Zubkova E. A., Tuchin V. V., Yaroslavsky I., Altshuler G. B. Optical clearing of human skin: comparative study of permeability and dehydration of intact and photothermally perforated skin // J. Biomed. Opt. 2008. Vol. 13, № 2. P. 021102.
  7. Andanson J. M., Hadgraft J., Kazarian S. G. In situ permeation study of drug through the stratum corneum using attenuated total refl ection Fourier transform infrared spectroscopic imaging // J. Biomed. Opt. 2009. Vol. 14, № 3. P. 034011.
  8. Williams A. C., Barry B. W. Penetration enhancers // Adv. Drug Deliv. Rev. 2004. Vol. 56. P. 603‒618.
  9. Cevc G., Vierl U. Nanotechnology and the transdermal route. A state of the art review and critical appraisal // J. Controlled Release. 2010. Vol. 141, № 3. P. 277‒299.
  10. Xu X., Zhu Q. Evaluation of skin optical clearing enhancement with Azone as a penetration enhancer // Optics Communications. 2007. Vol. 279. P. 223‒228.
  11. Jiang J., Wang R. K. How different molarities of oleic acid as enhancer exert its effect on optical clearing of skin tissue in vitro // J. X-Ray Science and Technology. 2005. Vol. 13. P. 149‒159.
  12. Jiang J., Boese M., Turner P., Wang R. K. Penetration kinetics of dimethyl sulphoxide and glycerol in dynamic optical clearing of porcine skin tissue in vitro studied by Fourier transform infrared spectroscopic imaging // J. Biomed. Opt. 2008. Vol. 13, № 2. P. 021105.
  13. Bui A. K., McClure R. A., Chang J., Stoianovici C., Hirshburg J., Yeh A. T., Choi B. Revisiting optical clearing with dimethyl sulfoxide (DMSO) // Lasers Surg. Med. 2009. Vol. 41. P. 142‒148.
  14. Sznitowska M. The infl uence of ethanol on permeation behavior of the porous pathway in the stratum corneum // Intern. J. Pharmacol. 1996. Vol. 137. P. 137‒140.
  15. Kurihara-Bergstrom T., Knutson K., de Noble L. J., Goates C. Y. Percutaneous absorption enhancement of an ionic molecule by ethanol–water system in human skin // Pharm. Res. 1990. Vol. 7. P. 762–766.
  16. Zhi Z., Han Z., Luo Q., Zhu D. Improve optical clearing of skin in vitro with propylene glycol as a penetration enhancer // J. Innov. Opt. Health Sci. 2009. Vol. 2, № 3. P. 269‒278.
  17. Zhong H., Guo Z., Wei H., Guo L., Wang C., He Y., Xiong H., Liu S. Synergistic effect of ultrasound and thiazone–PEG 400 on human skin optical clearing in vivo // Photochem. Photobiol. 2010. Vol. 86, № 3. P. 732–737.
  18. Chen K., Liang Y., Zhang Y. Study on refl ection of human skin with liquid paraffi n as the penetration enhancer by spectroscopy // J. Biomed. Opt. 2013. Vol. 18, № 10. P. 105001.
  19. Kai T., Mak V. H., Potts R. O., Guy R. H. Mechanism of percutaneous penetration enhancement: effect of n-alkanols on the permeability barrier of hairless mouse skin // Controlled Release. 1990. Vol. 12. P. 103‒112.
  20. Beastall J., Guy R.H., Hadgraft J., Wilding I. The infl uence of urea on percutaneous absorption // Pharmaceutical Research. 1986. Vol. 3, № 5. P. 294‒297.
  21. Alander J.T., Kaartinen I., Laakso A., Patila T., Spillmann T., Tuchin V. V., Venermo M., Valisuo P. A review of indocyanine green fl uorescent imaging in surgery // Intern. J. Biomedical Imaging. 2012. Vol. 2012. P. 940585.
  22. Park K. Y., Kim J. Y., Hyun M. Y., Oh W. J., Jeong S. Y., Han T. Y., Ahn J. Y., Kim B. J., Kim M. N. 1,213 Cases of treatment of facial acne using indocyanine green and intense pulsed light in Asian skin // BioMed Research International. 2015. Vol. 2015. P. 596161.
  23. Khosroshahi M. E., Nourbakhsh M. S., Saremi S., Tabatabaee F. Characterization of skin tissue soldering using diode laser and indocyanine green: in vitro studies // Lasers Med. Sci. 2010. Vol. 25, № 2. P. 207‒212.
  24. Mamoon A.-M., Gamal-Eldeen A. M., Ruppel M. E., Smith R. J., Tsang T., Miller L. M. In vitro effi ciency and mechanistic role of indocianine green as photodynamic therapy agent for human melanoma // Photodiagnosis and Photodynamic Therapy. 2009. Vol. 6. P. 105‒116.
  25. Mourant J. R., Johnson T. M., Los G., Bigio I. J. Noninvasive measurement of chemotherapy drug concentrations in tissue : preliminary demonstrations of in vivo measurements // Phys. Med. Biol. 1999. Vol. 44. P. 1397‒1417.
  26. Genina E. A., Bashkatov A. N., Tuchin V. V. Effect of ethanol on the transport of methylene blue through stratum corneum // Medical Laser Application. 2008. Vol. 23, № 1. P. 31‒38.
  27. Генина Э. А., Башкатов А. Н., Чикина Е. Э., Тучин В. В. Диффузия метиленового синего в слизистой оболочке верхнечелюстной пазухи человека // Биофизика. 2007. Т. 52, № 6. С. 1104‒1111.
  28. Mourant J. R., Bigio I. J., Jack D. A., Johnson T. M., Miller H. D. Measuring absorption coeffi cients in small volumes of highly scattering media : source-detector separations for which path lengths do not depend on scattering properties // Appl. Opt. 1997. Vol. 36, № 22. P. 5655‒5661.
  29. Генина Э. А., Башкатов А. Н., Тучин В. В. Исследование диффузии фотодинамического красителя индоци- анинового зеленого в коже с помощью спектроскопии обратного рассеяния // Квантовая электроника. 2014. Т. 44, № 7. С. 689‒695.
  30. Schaefer H., Redelmeier T.E. Skin Barrier. Principles of Percutaneous Absorption. Basel : Karger, 1996. 310 p.
  31. Barry B. W. Action of skin penetration enhancers – the lipid protein partitioning theory // Intern. J. Cosmetic Science. 1988. Vol. 10. P. 281‒293.
  32. Genina E. A., Bashkatov A. N., Sinichkin Yu. P., Kochubey V. I., Lakodina N. A., Altshuler G. B., Tuchin V. V. In vitro and in vivo study of dye diffusion into the human skin and hair follicles // J. Biomed. Opt. 2002. Vol. 7. P. 471‒477.
  33. Ghanem A. -H., Mahmoud H., Higuchi W. I., Liu P., Good W. R. The effects of ethanol on the transport of lipophilic and polar permeants across hairless mouse skin : Methods/validation of a novel approach // Intern. J. Pharm. 1992. Vol. 78. P. 137‒156.
  34. Mollee T. R., Bracken A. J. A Model of solute transport through stratum corneum using solute capture and release // Bull. of Mathematical Biology. 2007. Vol. 69. P. 1887–1907.
  35. Физические величины : справочник / под ред. И. С. Григорьева, Е. З. Мейлихова. М. : Энергоатомиздат, 1991. 1232 с.
  36. Котык А., Яначек К. Мембранный транспорт. М. : Мир, 1980. 341 с.
  37. Inamori T., Ghanem A.-H., Higuchi W. I., Srinivasan V. Macromolecules transport in and effective pore size of ethanol pretreated human epidermal membrane // Intern. J. Pharm. 1994. Vol. 105. P. 113‒123.
  38. Peck K. D., Ghanem A. -H., Higuchi W. I. Hindered diffusion of polar molecules through and effective pore radii estimates of intact and ethanol treated human epidermal membrane // Pharm. Res. 1994. Vol. 11. P. 1306‒1314.
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