Izvestiya of Saratov University.

Physics

ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)


For citation:

Doubrovski V. A., Markov S. V., Kovalev D. G. Red blood cells sedimentation as a collective process – experimental and theoretical modeling. Izvestiya of Sarat. Univ. Physics. , 2021, vol. 21, iss. 2, pp. 165-177. DOI: 10.18500/1817-3020-2021-21-2-165-177

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Published online: 
31.05.2021
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Language: 
Russian
Article type: 
Article
UDC: 
616-008.816:611.018.5

Red blood cells sedimentation as a collective process – experimental and theoretical modeling

Autors: 
Doubrovski Valerii Aleksandrovich, Saratov State Medical University named after V. I. Razumovsky
Markov Sergey Valerievich, Saratov State Medical University named after V. I. Razumovsky
Kovalev Dmitry Grigorievich, Saratov State Medical University named after V. I. Razumovsky
Abstract: 

Background and Objectives: The experimental and mathematical simulation was performed which aimed to study the mechanism of blood sedimentation process, which is the base of the standard medical diagnostic ESR method. Experimentally and by means of physical and mathematical simulation, the dependence of the blood solution sedimentation rate, as well as its model in the form of a porous mechanical disk on the parameters of both objects is studied. It was shown that the patterns of sedimentation of biological and mechanical objects are largely similar, that is a definite confirmation of the possibility to describe the blood sedimentation in the form of the collective red blood cell process previously proposed by the authors. Materials and Methods: The sedimentation rate definition method was based on digital frames analysis both for blood samples and mechanical discs. In experiments with blood 10×10×45 mm cuvettes were used. The whole blood concentration in samples is varied from 30% to 100%. The observation time for all samples was 120 minutes. In experiments with mechanical discs a vessel with 60 mm diameter was used. Discs have different parameters: the number and the size of holes. Results: Theoretical models for blood and disc sedimentation were created. The theoretical and experimental results are in agreement with each other. Moreover, both models show almost the same behavior. Conclusion: The presented theoretical and experimental results prove the conception of RBC sedimentation as a collective process which is important to acusto-optical blood typing method investigation.

Reference: 
  1. Hashemi R., Majidi A., Motamed H., Amini A., Najari F., Tabatabaey A. Erythrocyte sedimentation rate measurement using as a rapid alternative to the westergren method. Emergency, 2015, vol. 3, no. 2, pp. 50.
  2. Shi X., Lin G. Modeling the Sedimentation of Red Blood Cells in Flow under Strong External Magnetic Body Force Using a Lattice Boltzmann Fictitious Domain Method. Numer. Math. Theor. Meth. Appl., 2014, vol. 7, pp. 512–523. DOI: 10.4208/nmtma.2014.1306si
  3. Pribush A., Meyerstein D., Meyerstein N. The mechanism of erythrocyte sedimentation. Part 1: Channeling in sedimenting blood. Colloidsand Surfaces B: Biointerfaces, 2010, vol. 75. pp. 214 – 223. DOI: 10.1016/j.colsurfb.2009.08.036
  4. Ismailov R. M., Shevchuk N. A., Khusanov H. Mathematical model describing erythrocyte sedimentation rate. Implications for blood viscosity changes in traumatic shock and crush syndrome. BioMedical Engineering OnLine, 2005, vol. 4, no. 24. DOI: 10.1186/1475-925X-4-24
  5. Balakhovsky S. D. Reaktsiia osedaniia eritrotsitov [Reaction of RBC Sedimentation]. Moscow, Leningrad, GIZ Publ., 1928. 149 p. (in Russian).
  6. Doubrovski V. A., Dvoretski K. N., Markov S.V., Karpocheva E. P., Tuchin V. V. Optical digital registration of erythrocyte sedimentation and its modeling in the form of the collective process. Optics and Spectroscopy, 2019, vol. 126, no. 5, pp. 595–606.
  7. Doubrovski V. A., Dvoretski K. N. Ultrasonic wave action upon the red blood cell agglutination in vitro. Ultrasound in Medicine & Biology, 2000, vol. 26, no. 4, pp. 655–659. DOI: 10.1016/S0301-5629(99)00174-X
  8. Doubrovski V. A., Dvoretski K. N., Balaev A. E. Study of the mechanism of increased aggregation of erythrocytes by an ultrasound fi eld. Acustic Journal, 2004, iss. 50, no. 2, pp. 184–192 (in Russian).
  9. Dvoretski K. N. Uvelicheniye razreshayushchey sposobnosti fotometricheskogo metoda registratsii agglyutinatsii eritrotsitov cheloveka in vitro [Increasing the Resolution of the Photometric Method of Registration of the Agglutination of Human Erythrocytes in vitro]. Thesis Diss. Cand. Sci. (Biophys.). Saratov, 2005. 137 p (in Russian).
  10. Voeikov V. L. Physicochemical and physiological aspects of the erythrocyte sedimentation reaction. Uspekhi fi ziologicheskikh nauk, 1998, vol. 29, no. 4, pp. 55–73 (in Russian).
  11. Hung W. T., Collings A. F., Low J. Erythrocyte sedimentation rate studies in whole human blood. Phys. Med. Biol., 1994, vol. 39, no. 11, pp. 1855–1873.
  12. Fabry T. L. Mechanism of erythrocyte aggregation and sedimentation. Blood, 1987, Nov., vol. 70, no. 5, pp. 1572–1576.
Received: 
06.08.2020
Accepted: 
13.11.2020
Published: 
31.05.2021