Izvestiya of Saratov University.

Physics

ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)

For citation:

Darvin M. E., Choe C. S., Schleusener J. ., Lademann J. . Non-invasive Methods for in vivo Determination of the Skin Barrier Function – Advantages of Confocal Raman Microspectroscopy. Izvestiya of Saratov University. Physics , 2020, vol. 20, iss. 3, pp. 171-177. DOI: 10.18500/1817-3020-2020-20-3-171-177

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Published online: 
31.08.2020
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Language: 
English
Heading: 
Biophysics and medical physics
UDC: 
577.3
DOI: 
10.18500/1817-3020-2020-20-3-171-177

Non-invasive Methods for in vivo Determination of the Skin Barrier Function – Advantages of Confocal Raman Microspectroscopy

Autors: 
Darvin Maksim Evgen'yevich, Charité – Universitätsmedizin Berlin
Choe Chun Sik, Kim Il Sung University
Schleusener Johannes , Charité – Universitätsmedizin Berlin
Lademann Jürgen , Charité – Universitätsmedizin Berlin
Abstract: 

The physical barrier of the stratum corneum (SC) is provided by corneocytes and the lateral organization of intercellular lipids, which necessarily includes the orthorhombic organization phase. A review of methods used for in vivo non-invasive measurement of skin barrier function has been carried out and it has been shown that all currently used methods are indirect. The most popular method is the measurement of the transepidermal water loss (TEWL), which does not provide information on biophysical parameters responsible for the barrier function of the SC. It has been shown that confocal Raman microspectroscopy is the most suitable non-invasive method to determine the depth profile of the lateral organization of intercellular lipids, i.e. to study the skin barrier function in vivo.

Key words: 
intercellular lipids
lateral organization
orthorhombic organization
hexagonal organization
lipid lamella
stratum corneum
Raman spectroscopy
TEWL
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