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Darvin M. E., Choe C. S., Schleusener J. ., Lademann J. . Non-invasive Methods for in vivo Determination of the Skin Barrier Function – Advantages of Confocal Raman Microspectroscopy. //Izvestiya of Saratov University. New series. Series: Physics. , 2020, vol. 20, iss. 3, pp. 171-177. DOI:

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Non-invasive Methods for in vivo Determination of the Skin Barrier Function – Advantages of Confocal Raman Microspectroscopy

Darvin Maksim Evgen'yevich, Charité – Universitätsmedizin Berlin
Choe Chun Sik, Kim Il Sung University
Schleusener Johannes , Charité – Universitätsmedizin Berlin
Lademann Jürgen , Charité – Universitätsmedizin Berlin

The physical barrier of the stratum corneum (SC) is provided by corneocytes and the lateral organization of intercellular lipids, which necessarily includes the orthorhombic organization phase. A review of methods used for in vivo non-invasive measurement of skin barrier function has been carried out and it has been shown that all currently used methods are indirect. The most popular method is the measurement of the transepidermal water loss (TEWL), which does not provide information on biophysical parameters responsible for the barrier function of the SC. It has been shown that confocal Raman microspectroscopy is the most suitable non-invasive method to determine the depth profile of the lateral organization of intercellular lipids, i.e. to study the skin barrier function in vivo.

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