NEW SERIES. SERIES: PHYSICS

Izvestiya of Saratov University.

ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)


Cite this article as:

Darvin M. E., Choe C. S., Schleusener J. ., Lademann J. . Non-invasive Methods for in vivo Determination of the Skin Barrier Function – Advantages of Confocal Raman Microspectroscopy. //Izvestiya of Saratov University. New series. Series: Physics. , 2020, vol. 20, iss. 3, pp. 171-177. DOI: https://doi.org/10.18500/1817-3020-2020-20-3-171-177

Published online: 
31.08.2020
Language: 
English
UDC: 
577.3

Non-invasive Methods for in vivo Determination of the Skin Barrier Function – Advantages of Confocal Raman Microspectroscopy

Autors: 
Darvin Maksim Evgen'yevich, Charité – Universitätsmedizin Berlin
Choe Chun Sik, Kim Il Sung University
Schleusener Johannes , Charité – Universitätsmedizin Berlin
Lademann Jürgen , Charité – Universitätsmedizin Berlin
Abstract: 

The physical barrier of the stratum corneum (SC) is provided by corneocytes and the lateral organization of intercellular lipids, which necessarily includes the orthorhombic organization phase. A review of methods used for in vivo non-invasive measurement of skin barrier function has been carried out and it has been shown that all currently used methods are indirect. The most popular method is the measurement of the transepidermal water loss (TEWL), which does not provide information on biophysical parameters responsible for the barrier function of the SC. It has been shown that confocal Raman microspectroscopy is the most suitable non-invasive method to determine the depth profile of the lateral organization of intercellular lipids, i.e. to study the skin barrier function in vivo.

DOI: 
10.18500/1817-3020-2020-20-3-171-177
References: 
  1. Darvin M. E., Choe C. S., Schleusener J., Lademann J. Non-invasive depth profiling of the stratum corneum in vivo using confocal Raman microscopy considering the non-homogeneous distribution of keratin. Biomedical Optics Express, 2019, vol. 10, pp. 3092–3103.
  2. Schmitt T., Neubert R. H. H. State of the art in stratum corneum research. Part II: hypothetical stratum corneum lipid matrix models. Skin Pharmacology and Physiology, 2020. DOI: https://doi.org/10.1159/000509019
  3. Van Smeden J., Janssens M., Gooris G. S., Bouwstra J. A. The important role of stratum corneum lipids for the cutaneous barrier function. Biochimica et Biophysica Acta, 2014, vol. 1841, pp. 295–313.
  4. Choe C., Lademann J., Darvin M. E. Analysis of human and porcine skin in vivo / ex vivo for penetration of selected oils by confocal Raman microscopy. Skin Pharmacology and Physiology, 2015, vol 28, pp. 318–330.
  5. Nouveau-Richard S., Monot M., Bastien P., de Lacharriere O. In vivo epidermal thickness measurement: ultrasound vs. confocal imaging, Skin Research and Technology, 2004, vol. 10, pp. 136–140.
  6. Pilgram G. S. K., Engelsma-van Pelt A. M., Bouwstra J. A., Koerten H. K. Electron diffraction provides new information on human stratum corneum lipid organization studied in relation to depth and temperature. Journal of Investigative Dermatology, 1999, vol. 113, pp. 403–409.
  7. Kikuchi S., Aosaki T., Bito K., Naito S., Katayama Y. In vivo evaluation of lateral lipid chain packing in human stratum corneum. Skin Research and Technology, 2015, vol. 21, pp. 76–83.
  8. Choe C., Lademann J., Darvin M. E. A depth-dependent profi le of the lipid conformation and lateral packing order of the stratum corneum in vivo measured using Raman microscopy. Analyst, 2016, vol. 141, pp. 1981–1987.
  9. Yagi E., Sakamoto K., Nakagawa K. Depth dependence of stratum corneum lipid ordering: a slow-tumbling simulation for electron paramagnetic resonance, Journal of Investigative Dermatology, 2007, vol. 127, pp. 895–859.
  10. Doucet J., Potter A., Baltenneck C., Domanov Y. A. Micron-scale assessment of molecular lipid organization in human stratum corneum using microprobe X-ray diffraction. Journal of Lipid Research, 2014, vol. 55, pp. 2380–2388.
  11. Utz S. R., Karakaeva A. V., Galkina E. M. Noninvasive evaluation of the barrier properties of the skin (review). Saratov Journal of Medical Scientific Research, 2014, vol. 10, pp. 512–517.
  12. Antonov D., Schliemann S., Elsner P. Methods for the assessment of barrier function. Current Problems in Dermatology, 2016, vol. 49, pp. 61–70.
  13. Fluhr J. W., Feingold K. R., Elias P. M. Transepidermal water loss reflects permeability barrier status: validation in human and rodent in vivo and ex vivo models. Experimental Dermatology, 2006, vol. 15, pp. 483–492.
  14. Damien F., Boncheva M. The extent of orthorhombic lipid phases in the stratum corneum determines the barrier efficiency of human skin in vivo. Journal of Investigative Dermatology, 2010, vol. 130, pp. 611–614.
  15. Proksch E. pH in nature, humans and skin. Journal of Dermatology, 2018, vol. 45, pp. 1044–1052.
  16. Hanson K. M., Behne M. J., Barry N. P., Mauro T. M., Gratton E., Clegg R. M. Two-photon fluorescence lifetime imaging of the skin stratum corneum pH gradient. Biophysical Journal, 2002, vol. 83, pp. 1682–1690.
  17. Calzavara-Pinton P., Longo C., Venturini M., Sala R., Pellacani G. Refl ectance confocal microscopy for in vivo skin imaging. Photochemistry and Photobiology, 2008, vol. 84, pp. 1421–1430.
  18. Koehler M. J., Vogel T., Elsner P., Konig K., Buckle R., Kaatz M. In vivo measurement of the human epidermal thickness in different localizations by multiphoton laser tomography. Skin Research and Technology, 2010, vol. 16, pp. 259–264.
  19. Li W. J., Li P., Fang Y. H., Lei T., Dong K., Zou J., Gong W., Xie S. S., Huang Z. Quantitative assessment of skin swelling using optical coherence tomography. Photodiagnosis and Photodynamic Therapy, 2019, vol. 26, pp. 413–419.
  20. Boncheva M., Damien F., Normand V. Molecular organization of the lipid matrix in intact stratum corneum using ATR-FTIR spectroscopy. Biochimica et Biophysica Acta, 2008, vol. 1778, pp. 1344–1355.
  21. Van Logtestijn M. D., Caspers P. J., Kezic S., Hoffman D. R., Koenig D. W., Ono M., Stamatas G. N., Tanaka R. J. Water resistance profi le as a marker of skin barrier damage in atopic dermatitis patients. Journal of Dermatological Science, 2016, vol. 81, pp. 126–128.
  22. Choe C., Schleusener J., Lademann J., Darvin M. E. Age related depth profi les of human stratum corneum barrier-related molecular parameters by confocal Raman microscopy in vivo. Mechanisms of Ageing and Development, 2018, vol. 172, pp. 6–12.
  23. Choe C., Schleusener J., Lademann J., Darvin M. E. Human skin in vivo has a higher skin barrier function than porcine skin ex vivo – comprehensive Raman microscopic study of the stratum corneum. Journal of Biophotonics, 2018, vol. 11, pp. e201700355.
  24. Caussin J., Gooris G. S., Janssens M., Bouwstra J. A. Lipid organization in human and porcine stratum corneum differs widely, while lipid mixtures with porcine ceramides model human stratum corneum lipid organization very closely. Biochimica et Biophysica Acta, 2008, vol. 1778, pp. 1472–1482.
  25. Choe C., Schleusener J., Lademann J., Darvin M. E. In vivo confocal Raman microscopic determination of depth profiles of the stratum corneum lipid organization infl uenced by application of various oils. Journal of Dermatological Science, 2017, vol. 87, pp. 183–191.
Full text:
(downloads: 14)
Полный текст в формате PDF(En):
(downloads: 6)