Izvestiya of Saratov University.

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ISSN 2542-193X (Online)

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Tuchina E. S., Korchenova M. V., Zakoyan A. A., Tuchin V. V. Influence of strain differences on resistance of Staphylococcus aureus to photodynamic action using meso-substituted cationic porphyrins. Izvestiya of Saratov University. Physics , 2024, vol. 24, iss. 3, pp. 216-227. DOI: 10.18500/1817-3020-2024-24-3-216-227, EDN: IELOFE

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Published online: 
30.08.2024
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Russian
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Biophysics and medical physics
Article type: 
Article
UDC: 
577.344.3:57.033
DOI: 
10.18500/1817-3020-2024-24-3-216-227
EDN: 
IELOFE

Influence of strain differences on resistance of Staphylococcus aureus to photodynamic action using meso-substituted cationic porphyrins

Autors: 
Tuchina Elena S., Saratov State University
Korchenova Maria V., Saratov State University
Zakoyan Anna A., Research and Production Center “Armbiotechnology”
Tuchin Valery Viсtorovich, Saratov State University
Abstract: 

Background and Objectives. Infections associated with antibiotic-resistant strains of microorganisms, including Staphylococcus aureus, pose the greatest danger in nasopharyngeal diseases and post-surgical complications. A number of studies have shown that there are interstrain differences in the sensitivity of clinically significant microorganisms to the damaging effects of antimicrobial photodynamic therapy. In this work, in order to quantify interstrain differences in the responses of bacterial cells to photodynamic exposure, we studied the effectiveness of pyridyl porphyrin compounds in combination with LED radiation against three strains of Staphylococcus aureus. Materials and Methods. The objects of the study were: methicillin-sensitive museum strain S. aureus 209 P, methicillin-sensitive clinical strain S. aureus 5, methicillin-resistant clinical strain S. aureus 11. A LED with a maximum emission spectrum at a wavelength of λ = 405 nm and a half-width of 30 nm at a level of 0.1 of the maximum intensity, a power of 1.8 W and an integrated power density of 80 mW/cm2 was used as a radiation source. In all experiments, the radiation mode was continuous. The irradiation time varied from 5 to 30 min (irradiation doses from 24 to 144 J/cm2 , respectively). Water-soluble meso-substituted cationic pyridylporphyrins were used as photosensitizers: meso-tetrakis(N-(2’-hydroxyethyl)pyridinium-4-yl)porphyrin chloride (H2TOE4PyP) and its Zn(II) derivatives – zinc-meso-tetrakis [4-N-(2’-oxyethyl) pyridyl] porphyrin (Zn-TOE4PyP), zinc-meso-tetrakis [3-N-butyl pyridyl] porphyrin (Zn-TBut3PyP). To assess the level of oxidative stress and the tolerance of microorganisms to it, two different methods were used: 1) a method for determining the minimum inhibitory concentration of hydrogen peroxide, and 2) a method for determining the activity of bacterial catalase. Results and Discussion. The greatest sensitivity to the action of LED radiation was demonstrated by cells of the clinical methicillin-resistant strain S. aureus 11, activated by pyridyl porphyrins. It has been shown that when photosensitizers are used in concentrations of 0.01–0.03 mg/ml after 30 minutes of irradiation, a decrease in the number of cells of this strain occurs by 4.8 lgCFU/ml. It has been found that the activity of catalase in the cells of the methicillin-resistant strain S. aureus 11 is 17% lower compared to the activity of catalase in the cells of the standard strain S. aureus 209 P. This indirectly indicates the greater sensitivity of the strain S. aureus 11 to reactive oxygen species, formed during antimicrobial photodynamic exposure. Conclusion. It has been found that the differences in population reduction between strains range from 1.7 to 2.3 lgCFU/ml at the maximum irradiation dose, depending on the pyridylporphyrin modification used. It has been shown that the antibiotic-resistant strain S. aureus 11, which is highly sensitive to the action of ROS in the form of hydrogen peroxide and incapable of active production of catalase, is most susceptible to the complex action of LED radiation (405 nm) in combination with photosensitizers in the form of zinc- meso-tetrakis[3-N-butyl pyridyl]porphyrin (Zn-TBut3PyP).

Key words: 
antibacterial photodynamic therapy
LED radiation
405 nm
pyridyl porphyrins
Staphylococcus aureus
Acknowledgments: 
The authors express their gratitude to the National Academy of Sciences of the Republic of Armenia for the assistance provided within the framework of the Young Scientists Support Program (project No. 22-YSIP-010), as well as to the leading employee of the Bioengineering Laboratory of the G. Kh. Buniatyan Institute of Biochemistry of the NAS of Armenia (Yerevan, Armenia) G. V. Gyulhandanyan for providing the samples of pyridyl porphyrins; to the staff of the Department of Microbiology, Virology and Immunology of Saratov State Medical University named after V. I. Razumovsky (Saratov, Russia) for providing the strains of microorganisms; to the staff of the Department of Biochemistry and Biophysics of Saratov State University named after N. G. Chernyshevsky (Saratov, Russia) for assistance in conducting the experiments; to the employee of the Department of Optics and Biophotonics of Saratov State University named after N. G. Chernyshevsky (Saratov, Russia) L. E. Dolotov for assistance in conducting measurements and setting up the equipment. Funding Sources. The work was supported by the Ministry of Science and Higher Education of the Russian Federation (project No. 13.2251.21.0009 dated September 29, 2021 (Agreement No. 075-15-2021-942).
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Received: 
31.03.2024
Accepted: 
15.06.2024
Published: 
30.08.2024
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