Для цитирования:
Тучина Е. С., Корченова М. В., Закоян А. А., Тучин В. В. Влияние штаммовых различий на устойчивость Staphylococcus aureus к фотодинамическому воздействию с использованием мезо-замещенных катионных порфиринов // Известия Саратовского университета. Новая серия. Серия: Физика. 2024. Т. 24, вып. 3. С. 216-227. DOI: 10.18500/1817-3020-2024-24-3-216-227, EDN: IELOFE
Влияние штаммовых различий на устойчивость Staphylococcus aureus к фотодинамическому воздействию с использованием мезо-замещенных катионных порфиринов
В настоящей работе с целью количественного определения межштаммовых различий в реакциях бактериальных клеток на фотодинамическое воздействие было проведено исследование эффективности пиридилпорфириновых соединений в сочетании с светодиодным излучением в отношении трех штаммов Staphylococcus aureus.
Наибольшую чувствительность к действию излучения с длиной волны 405 нм, полушириной полосы 30 нм и плотностью мощности 80 мВт/см2 продемонстрировали клетки клинического метициллин-резистентногоштамма S. aureus 11, активированные пиридилпорфиринами. Показано, что при использовании фотосенсибилизаторов в концентрациях 0.01–0.03 мг/мл после 30 мин облучения снижение численности клеток данного штамма происходит на величину 4.8 lgКОЕ/мл. Установлено, что активность каталазы в клетках метициллинрезистентного штамма S. aureus 11 на 17% ниже по сравнению с активностью каталазы в клетках стандартного штамма S. aureus 209 Р. Это косвенно свидетельствует о большей чувствительности штамма S. aureus 11 к активным формам кислорода, образующимся в ходе антимикробного фотодинамического воздействия.
Полученные сведения имеют важное прикладное значение, поскольку показывают, что чувствительность к фотодинамическому воздействию клеток разных штаммов золотистого стафилококка различается в пределах 1.7–2.3 lgКОЕ/мл.
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