Izvestiya of Saratov University.

Physics

ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)


For citation:

Gamayunova E. А., Doronkina A. A., Lazareva E. N., Tuchina D. K., Kochubey V. I., Yanina I. I. Differences in optical properties of rat muscle tissue at room and physiological temperatures. Izvestiya of Saratov University. Physics , 2022, vol. 22, iss. 4, pp. 350-356. DOI: 10.18500/1817-3020-2022-22-4-350-356, EDN: MSQYVY

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Published online: 
30.11.2022
Full text:
(downloads: 99)
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Russian
Article type: 
Article
UDC: 
[612.014.462.7+612.014.462.8]:611.018.54+616.12:009.72
EDN: 
MSQYVY

Differences in optical properties of rat muscle tissue at room and physiological temperatures

Autors: 
Doronkina Anna A., Saratov State University
Lazareva Ekaterina Nikolaevna, Saratov State University
Tuchina Daria K., Saratov State University
Kochubey Vyacheslav Ivanovich, Saratov State University
Yanina Irina Iur'evna, Saratov State University
Abstract: 

Background and Objectives: Knowledge of the optical and thermal human tissues properties is essential for optimizing laser therapy and optical diagnostics. Most studies are carried out at room temperature of the sample. At the same time, it is known that the optical properties of biological tissues depend on temperature even in the physiological temperature range, that is, in the range of normal functioning of the body. Accordingly, there is a possibility that the use of literature data will lead to an incorrect assessment of the conditions for the propagation of light through biological tissue in a living object. Incorrect data on the optical properties of biological tissues at physiological temperature can lead to unreliable results in medical optical diagnostics or therapy. Therefore, studies of the temperature dependences of the optical properties of biological objects are undoubtedly topical. This work shows the differences in the optical properties of rat muscle tissue before and after heating the samples to physiological temperature. Materials and Methods: The spectra of collimated transmission, total transmission and diffuse reflection of rat muscle tissue were recorded. The spectra were recorded at 25°C (room temperature) and 38–39°C (physiological temperature). For each measured point of the sample, the spectral dependences of the absorption coefficients, transport scattering coefficient, and anisotropy factor of the studied samples were calculated. Results and conclusion: It has been shown that the optical properties of muscle tissue differ for room and physiological temperatures. In this case, the absorption and scattering coefficients practically do not change. The main parameter that changes with temperature is the anisotropy factor. This change also leads to a change in the transport scattering coefficient.

Acknowledgments: 
The study was supported by a grant Russian Science Foundation No. 21-72-10057, https://rscf.ru/project/21-72-10057/.
Reference: 
  1. Troy T. L., Thennadil S. N. Optical properties of human skin in the near infrared wavelength range of 1000 to 2200 nm. Journal of Biomedical Optics, 2001, vol. 6, iss. 2, pp. 167–176. https://doi.org/10.1117/1.1344191
  2. Laufer J., Simpson R., Kohl M., Cope M. Effect of temperature on the optical properties of ex vivo human dermis and subdermis. Phys. Med. Biol., 1998, vol. 43, pp. 2479–2489. https://doi.org/10.1088/0031-9155/43/9/004
  3. Troy T. L., Page D. L., Sevick-Muraca E. M. Optical properties of normal and diseased breast tissues: Prognosis for optical mammography. J. Biomed. Opt., 1996, vol. 1, pp. 342–355. https://doi.org/10.1117/12.239905
  4. Jaywant S., Wilson B., Patterson M., Lilge L., Flotte T. Temperature-dependent changes in the optical absorption and scattering spectra of tissues: Correlation with ultrastructure. Proc. SPIE. Laser-Tissue Interaction IV, 1993, vol. 1882. https://doi.org/10.1117/12.148080
  5. Nagarajan V. K., Yu B. Monitoring of Tissue Optical Properties During Thermal Coagulation of Ex Vivo Tissues. Lasers in Surgery and Medicine, 2016, vol. 48, pp. 686–694. https://doi.org/10.1002/lsm.22541
  6. Ao H. L., Xing D., Wei H. J., Gu H. M., Wu G. Y., Lu J. J. Thermal coagulation-induced changes of the optical properties of normal and adenomatous human colon tissues in vitro in the spectral range 400–1100nm. Phys. Med. Biol., 2008, vol. 53, pp. 2197–2206. https://doi.org/10.1088/0031-9155/53/8/013
  7. Thomsen S., Jacques S., Flock S. Microscopic correlates of macroscopic optical property changes during thermal coagulation of myocardium. Proc. Laser-Tissue Interaction, 1990, vol. 1202, iss. 2, pp. 11. https://doi.org/10.1117/12.17605
  8. Chung S. H., Cerussi A. E., Merritt S. I., Ruth J., Tromberg B. J. Non-invasive tissue temperature measurements based on quantitative DOS of water. Phys. Med. Biol., 2010, vol. 55, pp. 3753–3765. https://doi.org/10.1088/0031-9155/55/13/012
  9. Collins J. R. Change in the infra-red absorption spectrum of water with temperature. Phys. Rev., 1925, vol. 26, pp. 0771–0779. https://doi.org/10.1103/PhysRev.26.771
  10. Otal E. H., Inon F. A., Andrade F. J. Monitoring the temperature of dilute aqueous solutions using near-infrared water absorption. Appl. Spectrosc., 2003, vol. 57, pp. 661–6666. https://doi.org/10.1366/000370203322005355
  11. Kelly J. J., Kelly K. A., Barlow C. H. Tissue temperature by near-infrared spectroscopy. Proc. SPIE, 1995, vol. 2389, pp. 818–28. https://doi.org/10.1117/12.210025
  12. Libnau F. O., Kvalheim O. M., Christy A. A., Toft J. Spectra of water in the nearinfrared and midinfrared region. Vib. Spectrosc., 1994, vol. 7, pp. 243–254. https://doi.org/10.1016/0924-2031(94)85014-3
  13. Nachabé R., Hendriks B. H. W., Desjardins A. E., van der Voort Marjolein, Martin B. van der Mark. Estimation of lipid and water concentrations in scattering media with diffuse optical spectroscopy from 900 to 1600 nm. Journal of Biomedical Optics, 2010, vol. 15, no. 3, article no. 037015. https://doi.org/10.1117/1.3454392
  14. Merritt S. I. Combination of broadband diffuse optical spectroscopy with magnetic resonance imaging. PhD Thesis. University of California, Irvine, 2005. 160 p.
  15. Pimentel G. C., McClellan A. L. The Hydrogen Bond. San Francisco, W. Y. Freeman, 1960. 50 p.
  16. Chung S. H., Cerussi A. E., Klifa C., Baek H. M., Birgul O., Gulsen G., Merritt S. I., Hsiang D., Tromberg B. J. In vivo water state measurements in breast cancer using broadband diffuse optical spectroscopy. Phys. Med. Biol., 2008, vol. 53, pp. 6713–6727. https://doi.org/10.1088/0031-9155/53/23/005
  17. Buijs K., Choppin G. R. Near-infrared studies of structure of water. 1. Pure water. J. Chem. Phys., 1963, vol. 39, pp. 2035–2041. https://doi.org/10.1063/1.1734579
  18. Hollis V. S. Non-invasive monitoring of brain tissue temperature by near-infrared spectroscopy. PhD Thesis. University College, London, 2002. 263 p.
  19. Belov N. P., Lapshov S. N., Patyaev A. Yu., Sherstobitova A. S., Yaskov A. D. Temperature dependence of refraction index for ethylene glycol and propylene glycol aqueous solutions. Sci. Tech. J. Inf. Technol. Mech. Opt., 2012, no. 2 (78), pp. 138–139 (in Russian).
  20. Genina E. A., Bashkatov A. N., Kozintseva M. D., Tuchin V. V. OCT study of optical clearing of muscle tissue in vitro with 40% glucose solution. Optics and Spectroscopy, 2016, vol. 120, no. 1, pp. 20–27 (in Russian). https://doi.org/10.7868/S0030403416010098
  21. Bashkatov A. N., Berezin K. V., Dvoretskiy K. N., Chernavina M. L., Genina E. A., Genin V. D., Kochubey V. I., Lazareva E. N., Pravdin A. B., Shvachkina M. E., Timoshina P. A., Tuchina D. K., Yakovlev D. D., Yakovlev D. A., Yanina I. Yu., Zhernovaya O. S., Tuchin V. V. Measurement of tissue optical properties in the context of tissue optical clearing. J. Biomed. Opt., 2018, vol. 23, no. 9, article no. 091416. https://doi.org/10.1117/1.JB~O.23.9.091416
  22. Ghita A., Matousenk P., Stone N. Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues. Scientific Reports, 2018, no. 8, pp. 8379. https://doi.org/10.1038/s41598-018-25465-x
  23. International Guiding Principles for Biomedical Research Involving Animals. CIOMS and ICLAS. Available at: https://www.cioms.ch/index.php/12-newsflash/227-cioms-and-iclas-release-the-newinternational-guidingprinciples-for-biomedical-researchinvolving-animals (accessed 24 June 2022) (in Russian).
  24. Yanina I. Yu., Kozlova E. A., Kochubey V. I. Changes in the spectral characteristics of biological tissues depending on temperature. Proc. of SPIE, 2021, vol. 11641, article no. 116410X. https://doi.org/10.1117/12.2588231
  25. Inverse Adding-Doubling. Available at: https://omlc.org/software/iad/index.html (accessed 24 June 2022) (in Russian).
  26. Tuchin V. V. Optics of Biological Tissues. Methods of Light Scattering in Medical Diagnostics. Moscow, Fizmatlit Publ., 2013. 812 p. (in Russian).
Received: 
25.06.2022
Accepted: 
12.09.2022
Published: 
30.11.2022