For citation:
Agapov A. V., Bulavin M. V., Gayevsky V. N., Galushko A. V., Indyukova M. D., Mytsin G. V., Rzyanina A. V., Uglova S. S., Yamurzin V. R. Comparison of the radiosensitivity of a549 cells under gamma irradiation at the Rokus-M facility and at the channel No. 3 of the IBR-2 reactor. Izvestiya of Saratov University. Physics , 2026, vol. 26, iss. 2, pp. 149-157. DOI: 10.18500/1817-3020-2026-26-2-149-157, EDN: MORDBK
Comparison of the radiosensitivity of a549 cells under gamma irradiation at the Rokus-M facility and at the channel No. 3 of the IBR-2 reactor
Background and Objectives: The objectives of the study were to obtain data on survival and micronuclei formation in A549 lung carcinoma cells after exposure to induced γ-radiation at the IBR-2 channel No. 3 irradiation facility (Joint Institute for Nuclear Research, Dubna) with the reactor shut down and to compare the results with similar data obtained at the Rokus-M γ-therapeutic facility with a 60Co source. Materials and Methods: Human lung carcinoma A549 cells were exposed to γ-irradiation at the Rokus-M facility and channel No. 3 of the IBR-2 reactor with the reactor shut down. The radiation field characteristics from induced γ-radiation were measured, and survival and micronuclei formation in A549 lung carcinoma cells under these conditions were analyzed. Results: Data have been obtained on the characteristics of the radiation field from induced γ-radiation in the IBR-2 channel No. 3 irradiation unit with the reactor shut down and the extent of its influence on the radiosensitivity of A549 cells. A comparison of the identified radiosensitivity indices has been conducted with previously obtained data for the Rokus-M γ-therapeutic unit. Conclusions: Channel No. 3 of the IBR-2 with the reactor shut down can be used as an alternative γ-radiation source for biophysical experiments, since no statistically significant differences have been found between the frequency of micronuclei formation and cell survival during irradiation on the Rokus-M and IBR-2 units.
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