interference microscopy

Methods of Digital Holography in Interference Microscopy of Reflective Object in Partially Coherent Light

The theoretical foundation of the possibility of using the interference microscopes with extended and frequency broadband light source for recording digital holograms of microscopic objects, reconstruction and visualization of phase images is presented. Numerical procedure for processing of spatially localized digital holograms of the focused image in a partially coherent light are considered. Results of experiments on digital holographic microscopy of biological objects in a partially coherent light with Linnik interference microscope are shown.