Izvestiya of Saratov University.


ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)

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Kalinova A. E., Kuznetsova L. I., Ushakov A. V., Popova M. A., Abalymov A. A., Demina P. A., Anisimov R. A., Lomova M. V. Recrystallization of CaCO3 submicron magnetic particles in biological media. Izvestiya of Saratov University. Physics , 2023, vol. 23, iss. 4, pp. 371-377. DOI: 10.18500/1817-3020-2023-23-4-371-377, EDN: AKANPR

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Recrystallization of CaCO3 submicron magnetic particles in biological media

Kalinova Alexandra E., Saratov State University
Kuznetsova Ludmila Ivanovna, Saratov State University
Ushakov Arseni V., Saratov State University
Popova Maria A., Saratov State University
Abalymov Anatoliy A., Saratov State University
Demina Polina A., Saratov State University
Anisimov Roman A., Saratov State University
Lomova Maria V., Saratov State University

Background and Objectives: The development of magnetic theranostics is associated with the determination of the behavior of magnetic carriers in biosimilar media. In this work, we analyze the formation of different crystalline phases from magnetic mineral submicron calcium carbonate particles during incubation under conditions of cell cultivation in vitro for 3 days. The study of mineralmagneticsubmicron particles recrystallization was analyzed by XRD and electron scanning microscopy. The shape of calcium carbonate particles begins to change from elliptical to spherical under cell culture cultivations. As the amount of magnetite nanoparticle particles in calcium carbonate increases, the recrystallization process is faster with fallout of calcite, vaterite and magnetite phases. Materials and Methods: Scanning electron microscopy, processing of results using a self-written Python code, XRDwere utilized in this study. Results: The study of the process of recrystallization of magnetic mineral particles shows has shown that increasing the content of magnetic carriers leads to accelerated recrystallization of particles with simultaneous precipitation of calcite, vaterite and magnetite phases. Conclusion: Magnetic mineral submicron calcium carbonate particles are promising targets for theranostics with the self-destruction property in biological environments.

The work was supported by the Russian Science Foundation (project No. 23-13-00373, https://rscf.ru/project/23-13-00373/).
  1. Liu D., Yang F., Xiong F., Gu N. The Smart Drug Delivery System and Its Clinical Potential. Theranostics, 2016, vol. 6, iss. 9, pp. 1306–1323. https://doi.org/10.7150/thno.14858
  2. Ferreira A. M., Vikulina A. S., Volodkin D. V. CaCO3 Crystals as Versatile Carriers for Controlled Delivery of Antimicrobials. J. Controlled Release, 2020, vol. 328, pp. 470–489. https://doi.org/10.1016/j.jconrel.2020.08.061
  3. Kelkar S. S., Reineke T. M. Theranostics: Combining Imaging and Therapy. Bioconjugate Chemistry, 2011, vol. 22, iss. 10, pp. 1879–1903. https://doi.org/10.1021/bc200151q
  4. Sharma D., Ali A. A. E., Trivedi L. R. An Updated Review On: Liposomes as Drug Delivery System. Pharmatutor, 2018, vol. 6, iss. 2, pp. 50–62. https://doi.org/10.29161/PT.v6.i2.2018.50
  5. Fadia P., Tyagi S., Bhagat S., Nair A., Panchal P., Dave H., Dang S., Singh S. Calcium Carbonate Nano- and Microparticles: Synthesis Methods and Biological Applications. 3 Biotech., 2021, vol. 11, pp. 1–30. https://doi.org/10.1007/s13205-021-02995-2
  6. Liendo F., Arduino M., Deorsola F. A., Bensaid S. Factors Controlling and Influencing Polymorphism, Morphology and Size of Calcium Carbonate Synthesized through the Carbonation Route: A Review. Powder Technol., 2022, vol. 398, no. 117050. https://doi.org/10.1016/j.powtec.2021.117050
  7. Goswami M. M., Dey C., Bandyopadhyay A., Sarkar D., Ahir M. Micelles Driven Magnetite (Fe3O4) Hollow Spheres and a Study on AC Magnetic Properties for Hyperthermia Application. Journal of Magnetism and Magnetic Materials, 2016, vol. 417, pp. 376–381. https://doi.org/10.1016/j.jmmm.2016.05.069
  8. Feoktistova N. A., Vikulina A. S., Balabushevich N. G., Skirtach A. G., Volodkin D. Bioactivity of Catalase Loaded into Vaterite CaCO3 Crystals via Adsorption and Co-Synthesis. Materials & Design, 2020, vol. 185, article no. 108223. https://doi.org/10.1016/j.matdes.2019.108223
  9. Wu C., Liu X., Yao F., Yang X., Wang Y., Hu W. Crystalline-Magnetism Action in Biomimetic Mineralization of Calcium Carbonate. Chinese Journal of Chemical Engineering, 2023, vol. 59, pp. 146–152. https://doi.org/10.1016/j.cjche.2023.01.004
  10. Ponomar V. Crystal Structures and Magnetic Properties of Spinel Ferrites Synthesized from Natural Fe–Mg– Ca Carbonates. Materials Research Bulletin, 2023, vol. 158, article no. 112068. https://doi.org/10.1016/j.materresbull.2022.112068
  11. Fakhrullin R. F., Bikmullin A. G., Nurgaliev D. K. Magnetically Responsive Calcium Carbonate Microcrystals. ACS Applied Materials & Interfaces, 2009, vol. 1, iss. 9, pp. 1847–1851. https://doi.org/10.1021/am9003864
  12. German S. V., Inozemtseva O. A., Markin A. V., Metvalli Kh., Khomutov G. B., Gorin D. A. Synthesis of Magnetite Hydrosols in Inert Atmosphere. Colloid Journal, 2013, vol. 75, iss. 4, pp. 483–486. https://doi.org/10.1134/S1061933X13040042
  13. Kozlova A. A., German S. V., Atkin V. S., Zyev V. V., Astle M. A., Bratashov D. N., Svenskaya Y. I., Gorin D. A. Magnetic Composite Submicron Carriers with Structure-Dependent MRI Contrast. Inorganics, 2020, vol. 8, iss. 2, article no. 11. https://doi.org/10.3390/inorganics8020011
  14. German S. V., Novoselova M. V., Bratashov D. N., Demina P. A., Atkin V. S., Voronin D. V., Khlebtsov B. N., Parakhonskiy B. V., Sukhorukov G. B., Gorin D. A. High-Efficiency Freezing-Induced Loading of Inorganic Nanoparticles and Proteins into Micron- and Submicron-Sized Porous Particles. Scientific Reports, 2018, vol. 8, iss. 1, article no. 17763. https://doi.org/10.1038/s41598-018-35846-x
  15. Atchudan R., Perumal S., Joo J., Lee Y. R. Synthesis and Characterization of Monodispersed Spherical Calcium Oxide and Calcium Carbonate Nanoparticles via Simple Pyrolysis. Nanomaterials, 2022, vol. 12, iss. 14, article no. 2424. https://doi.org/10.3390/nano12142424