Izvestiya of Saratov University.


ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)

For citation:

Khomutov E. V., Dmitriev L. S., Potapov V. V., Zenin O. K., Zaitsev S. Y. Physical-chemical approach to the calculation of adsorption characteristics of low molecular components of blood of the patients with heart failure. Izvestiya of Sarat. Univ. Physics. , 2022, vol. 22, iss. 3, pp. 244-253. DOI: 10.18500/1817-3020-2022-22-3-244-253

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Published online: 
Full text:
(downloads: 66)
Article type: 

Physical-chemical approach to the calculation of adsorption characteristics of low molecular components of blood of the patients with heart failure

Khomutov Evgeni V., Donetsk National Medical University named after M. Gorky
Dmitriev Leonid S., Donetsk National Medical University named after M. Gorky
Potapov Vladimir Vladimirovich, Donetsk National Medical University named after M. Gorky
Zenin Oleg K., Penza State University
Zaitsev Sergei Yu., Moscow State University of Food Production

Background and Objectives: Despite the fact that the methods of dynamic interfacial tensiometry and rheology are used to study blood and other biological fluids, in medicine there is no unified approach in the methodology of analysis and interpretation of the results. However, in some cases, the obtained numerical characteristics do not have a simple physical meaning and do not allow to evaluate the contribution of individual components and, as a consequence, it is impossible to identify the main link. Taking into account the multidirectional and nongeneralized data on rheology and due to the multicomponent and uncertain composition, there have been no successful attempts so far to create models to determine the real contribution of individual blood components. The purpose of this study is to develop a model, based on which one could obtain information about physical constants and parameters of individual plasma and/or blood serum components for medical diagnostics. Materials and Methods: The study is performed on blood samples of two human groups: group 1) 15 patients with coronary heart disease aged from 51 to 76 years; group 2) conditionally healthy volunteers (15 people) aged from 50 to 75 years. Results: For serum/plasma fractionation, a protein “desalting” method is used to obtain a fraction of low-molecular-weight metabolites. Further determination of interphase tensiometry is performed by the hanged drop method using hardware-software complex PAT-1 (“Sinterface Technologies”, Germany). The curves of the dynamic interfacial tension of the studied groups differ significantly throughout the entire time of the experiment. The average values of the dynamic interfacial tension of native serum in group 1 are noticeably lower than in the group 2 (the group of healthy volunteers). The detected change in the value of the dynamic interfacial tension may indicate an accumulation of surfactants in the serum of patients with coronary heart disease. The data obtained are in satisfactory agreement withthemathematicalmodel proposed bythe authors. Ifthe adsorption atthe beginning of the experiment is due mainly to low molecular weight compounds, then the value of the sum of the concentrations of high molecular weight compounds (after protein precipitation) should be slightly lower than the values of the corresponding indicators of the native blood samples. Conclusion: Using the obtained data of dynamic surface tension and the proposed mathematical model the following conclusions have been formulated: 1) tensiometric characteristics of blood serum for group 1 and 2 differ in dynamic and equilibrium surface tension; 2) the quantitative and qualitative contribution of the coagulation system to surface tension and the sum of concentrations of low molecular weight compounds; 3) the contribution of low molecular weight metabolites in the formation of surface tension of plasma and serum has been estimated; 4) the heterogeneity of the qualitative composition of low-molecular substances fractions in the studied groups has been shown.

The work on section 1.2 was carried out within the framework of the task of the research laboratory of animal ophthalmology, oncology and biochemistry, Moscow State University of Food Production (Registration number: 1022060100048-0-4.3.1).
  1. Kazakov V. N. Mezhfaznaya tenziometriya i reometriya biologicheskikh zhidkostey v terapevticheskoy praktike [Dynamic Surface Tension of Biological Fluids in Medicine]. Donetsk, Donetsk Medical University Publ., 2000. 296 p. (in Russian).
  2. Kazakov V., Vozianov A., Sinyachenko O., Trukhin D., Kovalchuk V. Studies on the application of dynamic surface tensiometry of serum and cerebrospinal liquid for diagnostics and monitoring of treatment in patients who have rheumatic, neurological or oncological diseases. Advances in Colloid and Interface Science, 2000, vol. 86, no. 1–2, pp. 1–38. https://doi.org/10.1016/s0001-8686(00)00031-2
  3. Zaitsev S. Dynamic surface tension measurements as general approach to the analysis of animal blood plasma and serum. Advances in Colloid and Interface Science, 2016, vol. 235, pp. 201–213. https://doi.org/10.1016/j. cis.2016.06.007
  4. Gálvez-Ruiz M. Different approaches to study protein films at air/water interface. Advances in Colloid and Interface Science, 2017, vol. 247, pp. 533–542. https://doi.org/10.1016/j.cis.2017.07.015
  5. Agassandian M., Mallampalli R. Surfactant phospholipid metabolism. Biochimica et Biophysica Acta (BBA). Molecular and Cell Biology of Lipids, 2013, vol. 1831, no. 2, pp. 612–625. https://doi.org/10.1016/j.bbalip.2012.09.010
  6. Dutcher C., Wexler A., Clegg S. Surface Tensions of Inorganic Multicomponent Aqueous Electrolyte Solutions and Melts. The Journal of Physical Chemistry A, 2010, vol. 114, no. 46, pp. 12216–12230. https://doi.org/10.1021/jp105191z
  7. Muraviev A. V., Tikhomirova I. A., Bulaeva S. V. Study of the role of individual rheological characteristics of blood in changing its fluidity and transport potential. Rossiyskiy zhurnal biomekhaniki [Russian Journal of Biomechanics], 2012, vol. 3, pp. 32–41 (in Russian).
  8. Fathi Azarbayjani A., Jouyban A. Surface tension in human pathophysiology and its application as a medical diagnostic tool. BioImpacts, 2015, vol. 5, no. 1, pp. 29– 44. https://doi.org/10.15171/bi.2015.06
  9. Kuznetsova I. V., Potapov V. V., Shramenko E. K., Zenin O. K. Surface tension and dilation viscoelasticity of blood serum in patients with ischemic heart disease, operated in the conditions of artificial blood circulation. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Meditsinskiye nauki [University Proceedings. Volga Region. Medical Sciences], 2019, no. 3 (51), pp. 140–149 (in Russian). https://doi.org/10.21685/2072-3032-2019-3-13
  10. Zaitsev S. Yu. Tenziometricheskiy i biokhimicheskiy analiz krovi zhivotnykh : fundamental’nyye i prikladnyye aspekty [Tensiometric and Biochemical Analysis of Animal Blood : Fundamental and Applied Aspects]. Moscow, Izdatel’stvo “Sel’skokhozyaistvennye tekhnologii”, 2016. 192 p. (in Russian).
  11. Ernst E., Resch K., Matrai A., Buhl M., Schlosser P., Paulsen H. Impaired blood rheology : A risk factor after stroke? Journal of Internal Medicine, 1991, vol. 229, no. 5, pp. 457–462. https://doi.org/10.1111/j.1365-2796.1991.tb00375.x
  12. Ernst E., Krauth U., Resch K., Paulsen H. Does blood rheology revert to normal after myocardial infarction? Heart, 1990, vol. 64, no. 4, pp. 248–250. https://doi.org/10.1136/hrt.64.4.248
  13. Moöbius D., Miller R. Proteins at liquid interfaces. Studies in Interface Science, 1998, vol. 7, pp. 1–498.
  14. Shrestha L., Matsumoto Y., Ihara K., Aramaki K. Dynamic Surface Tension and Surface Dilatational Elasticity Properties of Mixed Surfactant/Protein Systems. Journal of Oleo Science, 2008, vol. 57, no. 9, pp. 485– 494. https://doi.org/10.5650/jos.57.485
  15. Zaitsev S. Yu., Bogolyubova N. V., Zhang X., Brenig B. Biochemical parameters, dynamic tensiometry and circulating nucleic acids for cattle blood analysis : A review. PeerJ, 2020, vol. 8, article e8997. https://doi.org/10.7717/peerj.8997
  16. Ishchuk T., Raetska Y., Savchuk O., Ostapchenko L. Changes in blood protein composition under experimental chemical burns of esophageal development in rats. Biomedical Research and Therapy, 2015, vol. 2, no. 4, pp. 241–249. https://doi.org/10.7603/s40730-015-0009-x
  17. Lyakh Yu. E., Gur’yanov V. G., Khomenko V. N., Panchenko O. A. Osnovy komp’yuternoy biostatistiki : analiz informatsii v biologii, meditsine i farmatsii statisticheskim paketom [Fundamentals of Computer Biostatistics : Analysis of Information in Biology, Medicine and Pharmacy Using the MedStat Statistical Package]. Donetsk, Donetsk Medical University Publ., 2006. 214 p. (in Russian).
  18. Attinger D., Moore C., Donaldson A., Jafari A., Stone H. Fluid dynamics topics in bloodstain pattern analysis : Comparative review and research opportunities. Forensic Science International, 2013, vol. 231, no. 1–3, pp. 375–396. https://doi.org/10.1016/j.forsciint.2013.04. 018
  19. Makievski A., Loglio G. Krägel J., Miller R., Fainerman V., Neumann A. Adsorption of Protein Layers at the Water / Air Interface As Studied by Axisymmetric Drop and Bubble Shape Analysis. The Journal of Physical Chemistry B, 1999, vol. 103, no. 44, pp. 9557–9561. https://doi.org/org/10.1021/jp990775y
  20. Kairaliyeva T., Aksenenko E., Mucic N., Makievski A., Fainerman V., Miller R. Surface Tension and Adsorption Studies by Drop Profile Analysis Tensiometry. Journal of Surfactants and Detergents, 2017, vol. 20, no. 6, pp. 1225–1241. https://doi.org/10.1007/s11743-017-2016-y
  21. Fainerman V., Trukhin D., Zinkovych I., Miller R. Interfacial tensiometry and dilational surface visco-elasticity of biological liquids in medicine. Advances in Colloid and Interface Science, 2018, vol. 255, pp. 34–46. https://doi.org/10.1016/j.cis.2017.08.002
  22. Moiseeva I. Ya., Potapov V. V., Zenin O. K., Kuznetsova I. V., Dmitriev L. S. Rheological parameters of blood serum and plasma in patients operated on the heart under cardiopulmonary bypass in the periand intraoperating period. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Meditsinskiye nauki [University Proceedings. Volga Region. Medical Sciences], 2020, no. 1 (53), pp. 129–154 (in Russian). https://doi.org/10.21685/2072-3032-2020-1-14
  23. Kamenev V. F., Strelnikova I. L., Maslennikov A. A. Comparative characteristics of hemostasis system of aorta and veins in patients with chronic cardiac insufficiency caused by chronic cardiac insufficiency. Scientific Bulletins of Belgorod State University. Series : Medicine. Pharmacia, 2012, No. 4 (123), iss. 17, pp. 81– 84 (in Russian).
  24. Bulashova O. V., Malkova M. I. Significance of D-dimer in the diagnosis and prognosis of thromboembolic complications in cardiac patients. Prakticheskaya meditsina [Practical Medicine], 2012, vol. 5 (60), pp. 81–84 (in Russian).
  25. Song B., Shu Y., Xu Y. N., Fu P. Plasma fibrinogen lever and risk of coronary heart disease among Chinese population : A systematic review and metaanalysis. International Journal of Clinical and Experimental Medicine, 2015, vol. 8, no. 8, pp. 13195–13202. https://doi.org/10.1186/s13293-018-0210-x
  26. Karbaschi M., Bastani D., Javadi A., Kovalchuk V., Kovalchuk N., Makievski A. Drop profile analysis tensiometry under highly dynamic conditions. Colloids and Surfaces A : Physicochemical and Engineering Aspects, 2012, vol. 413, pp. 292–297. https://doi.org/org/10.1016/j.colsurfa.2012.04.027
  27. Dementieva I. I., Roytman E. V. Diagnostic significance of blood rheology studies during cardiac surgery. Anesteziologiya i reanimatologiya [Anesthesiology and Resuscitation], 1999, vol. 5, pp. 25–28 (in Russian).
  28. Morange P., Bickel C., Nicaud V., Schnabel R., Rupprecht H., Peetz D. Haemostatic Factors and the Risk of Cardiovascular Death in Patients With Coronary Artery Disease. Arteriosclerosis, Thrombosis, and Vascular Biology, 2006, vol. 26, no. 12, pp. 2793–2799. https://doi.org/10.1161/01.ATV.0000249406.92992.0d