Izvestiya of Saratov University.

Physics

ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)

For citation:

Selifonov A. A., Rykhlov A. S., Tuchin V. V. Ex vivo study of the kinetics of ovarian tissue optical properties under the influence of 40%-glucose. Izvestiya of Saratov University. Physics , 2023, vol. 23, iss. 2, pp. 120-127. DOI: 10.18500/1817-3020-2023-23-2-120-127, EDN: SHGJIM

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Published online: 
30.06.2023
Full text:
download
(downloads: 163)
Language: 
English
Heading: 
Biophysics and medical physics
Article type: 
Article
UDC: 
535.341.08:535.346.1
DOI: 
10.18500/1817-3020-2023-23-2-120-127
EDN: 
SHGJIM

Ex vivo study of the kinetics of ovarian tissue optical properties under the influence of 40%-glucose

Autors: 
Selifonov Alexey Andreevich, Education and Research Institute of Nanostructures and Biosystems, Saratov State University
Rykhlov Andrey Sergeevich, Clinic “Veterinary Hospital”, Saratov State University of Genetics, Biotechnology and Engineering named after N. I. Vavilov
Tuchin Valery Viсtorovich, Saratov State University
Abstract: 

Background and Objectives: The reproductive system of women is a subject of diverse multidisciplinary research worldwide. These are oncological diseases, infertility of an unspecified nature, ovarian cryopreservation to preserve fertility, improvement of early diagnosis of diseases, etc. Elucidation of the mechanisms of diffusion of tissue water and hyperosmotic agents in luteal phase ovarian tissues controlled by аngiogenic growth factors can bring us closer to understanding biophysical processes in general. Materials and Methods. The work used spectroscopy of diffuse reflection, a free diffusion model and the modified Bouguer–Lambert–Beer law. Results. The diffusion coefficient of 40% glucose/tissue water intothe ovariantissue ofthe luteal phase D andthe diffusion time τ have been determined, which was D = (8.6 ± 1.4)·107 cm2 /s and τ = 50.4 ± 1.7 min with a samplethickness of(0.8 ± 0.1)mm. The efficiency of optical clearing of cat ovariantissues with 40% glucose immersion has been determined. Conclusions. Studies have shown that 40%-glucose is an effective optical clearing agent for topical use in differentiating normal and pathological ovarian tissues and in clinical applications.

Key words: 
ovarian tissues
luteal phase
glucose
total transmittance spectra
diffuse reflectance spectra
diffusion coefficient
optical clearing efficiency
Acknowledgments: 
The study was financially supported by the Russian Science Foundation under project No. 22-75-00021 dated July 28, 2022.
Reference: 
  1. Pervaiz S., Naeem M., Ali A., John А., Batool N. Frequency of uterine anomalies associated with persistent miscarriages in pregnancy on ultrasound: Uterine anomalies associated with persistent miscarriages. Pakistan Journal of Health Sciences, 2022, vol. 4, pp. 55–58. https://doi.org/10.54393/pjhs.v3i01.54
  2. Tuchin V. V. Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnostics. 3rd ed. Bellingham, SPIE Press, 2015. 988 p.
  3. Nian L., Yang D-H., Zhang J., Zhao H., Zhu C-F., Dong M-F., Ai Y. Analysis of the clinical efficacy of laparoscopy and hysteroscopy in the treatment of tubal-factor infertility. Front. Med., 2021, vol. 7, article no. 712222. https://doi.org/10.3389/fmed.2021.712222
  4. Khan M. H., Choi B., Chess S., Kelly K. M., McCullough D., Nelson D. S. Optical clearing of in vivo human skin: Implications for light-based diagnostic imaging and therapeutics. Lasers in Surgery and Medicine, 2004, vol. 34, no. 2, pp. 83–85.
  5. Tuchin V. V. Optical Clearing of Tissues and Blood. SPIE Press, Bellingham, USA, 2006. 254 p.
  6. Oliveira L. M. C., Tuchin V. V. The Optical Clearing Method – A New Tool for Clinical Practice and Biomedical Engineering. Springer, Cham, Switzerland, 2019. 188 p.
  7. Tuchina D. K., Timoshina P. A., Tuchin V. V., Bashkatov A. N., Genina E. A. Kinetics of Rat Skin Optical Clearing at Topical Application of 40% Glucose: Ex Vivo and In Vivo Studies. IEEE Journal of Selected Topics in Quantum Electronics, 2019, vol. 25, no. 1, pp. 1–8. https://doi.org/10.1109/JSTQE.2018.2830500
  8. Oliveira L. R., Ferreira R. M., Pinheiro M. R., Silva H. F., Tuchin V. V., Oliveira L. M. Broadband spectral verification of optical clearing reversibility in lung tissue. Journal of Biophotonics, 2023, vol. 16, no. 1, pp. e202200185. https://doi.org/10.1002/jbio.202200185
  9. Carneiro I., Carvalho S., Henrique R., Selifonov A., Oliveira L., Tuchin V. V. Enhanced ultraviolet spectroscopy by optical clearing for biomedical applications. IEEE Journal of Selected Topics in Quantum Electronics, 2021, vol. 27, no. 4, pp. 1–8. https://doi.org/10.1109/JSTQE.2020.3012350
  10. Tuchin V. V., Zhu D., Genina E. A. Handbook of Tissue Optical Clearing: New Prospects in Optical Imaging. Boca Raton, CRC Press, 2022. 688 p.
  11. Lee S., Ozkavukcu S., Ku S. Y. Current and future perspectives for improving ovarian tissue cryopreservation and transplantation outcomes for cancer patients. Reproductive Sciences, 2021, vol. 28, no. 6, pp. 1746–1758. https://doi.org/10.1007/s43032-021-00517-2
  12. Abbara A., Jayasena C. N., Christopoulos G., Narayanaswamy S., Izzi-Engbeaya C., Nijher G. M. K., Comninos A. N., Peters D., Buckley A., Ratnasabapathy R., Prague J. K., Salim R., Lavery S. A., Bloom S. R., Szigeti M., Ashby D. A., Trew G. H., Dhillo W. S. Efficacy of kisspeptin-54 to trigger oocyte maturation in women at high risk of ovarian hyperstimulation syndrome (OHSS) during in vitro fertilization (IVF) therapy. Journal of Clinical Endocrinology and Metabolism, 2015, vol. 100, no. 9, pp. 3322–3331. https://doi.org/10.1210/jc.2015-2332
  13. Burkman R. T. Atlas of pelvic anatomy and gynecologic surgery. The Journal of the American Medical Association, 2011, vol. 306, no. 18, pp. 2031–2038. https://doi.org/10.1001/jama.2011.1635
  14. Smith M. F., McIntush E. W., Smith G. W. Mechanisms associated with corpus luteum development. Journal of Animal Science, 1994, vol. 72, no. 7, pp. 1857–1872. https://doi.org/0.2527/1994.7271857x
  15. Genina E. A., Bashkatov A. N., Tuchin V. V. Tissue optical immersion clearing. Expert Review of Medical Devices, 2010, vol. 7, no. 6, pp. 825–842. https://doi.org/10.1586/erd.10.50
  16. Worne F. Color atlas of veterinary anatomy. Volume 3: The dog and cat. Australian Veterinary Journal, 2011, vol. 89, no. 5, pp. 124–136. https://doi.org/10.1111/j.1751-0813.2010.00675
  17. Carvalho S., Gueiral N., Nogueira E., Henrique R., Oliveira L., Tuchin V. V. Glucose diffusion in colorectal mucosa – a comparative study between normal and cancer tissues. Journal of Biomedical Optics, 2017, vol. 22, no. 9, pp. 91506. https://doi.org/10.1117/1.JBO.22.9.091506
  18. Selifonov A. A., Tuchin V. V. Determination of the diffusion coefficient of 40%-glucose in human gum tissue by optical method. Optics and Spectroscopy, 2020, vol. 128, no. 6, pp. 766–770.
  19. Carneiro I., Carvalho S., Henrique R., Oliveira L., Tuchin V. V. Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue. Journal of Biomedical Optics, 2017, vol. 22, no. 12, рр. 1–10. https://doi.org/10.1117/1.JBO.22.12.125002
Received: 
23.12.2022
Accepted: 
20.03.2023
Published: 
30.06.2023
  • 708 reads