Izvestiya of Saratov University.

Physics

ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)

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Selifonov A. A., Rykhlov A. S., Tuchin V. V. Ex vivo study of the kinetics of ovarian tissue optical properties under the influence of 40%-glucose. Izvestiya of Saratov University. Physics , 2023, vol. 23, iss. 2, pp. 120-127. DOI: 10.18500/1817-3020-2023-23-2-120-127, EDN: SHGJIM

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Published online: 
30.06.2023
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Language: 
English
Heading: 
Biophysics and medical physics
Article type: 
Article
UDC: 
535.341.08:535.346.1
DOI: 
10.18500/1817-3020-2023-23-2-120-127
EDN: 
SHGJIM

Ex vivo study of the kinetics of ovarian tissue optical properties under the influence of 40%-glucose

Autors: 
Selifonov Alexey Andreevich, Education and Research Institute of Nanostructures and Biosystems, Saratov State University
Rykhlov Andrey Sergeevich, Clinic “Veterinary Hospital”, Saratov State University of Genetics, Biotechnology and Engineering named after N. I. Vavilov
Tuchin Valery Viсtorovich, Science Medical Center, Saratov State University
Abstract: 

Background and Objectives: The reproductive system of women is a subject of diverse multidisciplinary research worldwide. These are oncological diseases, infertility of an unspecified nature, ovarian cryopreservation to preserve fertility, improvement of early diagnosis of diseases, etc. Elucidation of the mechanisms of diffusion of tissue water and hyperosmotic agents in luteal phase ovarian tissues controlled by аngiogenic growth factors can bring us closer to understanding biophysical processes in general. Materials and Methods. The work used spectroscopy of diffuse reflection, a free diffusion model and the modified Bouguer–Lambert–Beer law. Results. The diffusion coefficient of 40% glucose/tissue water intothe ovariantissue ofthe luteal phase D andthe diffusion time τ have been determined, which was D = (8.6 ± 1.4)·107 cm2 /s and τ = 50.4 ± 1.7 min with a samplethickness of(0.8 ± 0.1)mm. The efficiency of optical clearing of cat ovariantissues with 40% glucose immersion has been determined. Conclusions. Studies have shown that 40%-glucose is an effective optical clearing agent for topical use in differentiating normal and pathological ovarian tissues and in clinical applications.

Key words: 
ovarian tissues
luteal phase
glucose
total transmittance spectra
diffuse reflectance spectra
diffusion coefficient
optical clearing efficiency
Acknowledgments: 
The study was financially supported by the Russian Science Foundation under project No. 22-75-00021 dated July 28, 2022.
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Received: 
23.12.2022
Accepted: 
20.03.2023
Published: 
30.06.2023
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