Biophysics and medical physics

Influence of the Hydrogen Bond on the IR-spectrum and Structure of Molecular Complex of Diamond Nanoparticles and DNA Bases

Background and Objectives: Using molecular modeling by the density functional theory method we analyze a hydrogen bonds formation and their influence on IR-spectrum and structure of molecular complex which is formed as the interaction of complementary couple of DNA nucleobases adenine and timin and nanodiamonds surrounded with carboxylic groups. As an example of nanodiamonds adamantine has been used. Intermolecular forces and structure of hydrogen bonds are investigated.

Investigation of Mildronat ® Diffusion in Human Eye Sclera

Background and Objectives: Diseases of retina and optic nerve are the major causes of vision loss. The use of new drugs in the treatment of these diseases can reduce the decline of visual function due to the involvement of additional mechanisms of cell metabolism compensation. Thus, investigation of the drug diffusion in sclera is an important task at estimation of dose necessary for achievement of enough drug concentration in internal eye tissues.

Processing and Visualization of Large Data Arrays in Taxonomic and Evolutionary Studies of Living Nature1 (a review)

A review is given of recent advances in the taxonomic study of organisms and current views on biological evolution and the origin of life. The steady increase in bioinformation resources is noted, which reflects the results of studies of the Earth’s biodiversity with the use of deciphered structures of biomacromolecules (DNA, RNA, proteins, etc.). This necessitates accounting for the specific character of manipulations with large data arrays, which is currently termed the big data problem.

Study of Ethanol Impact on the Transepidermal Transport of Indocyanine Green with Backscattering Spectroscopy

In the work, impact of aqueous 40%-ethanol solution on the transepidermal transport of photodynamic dye indocyanine green has been studied with backscattering spectroscopy. It has been first obtained the value of the dye diffusion coefficient through the stratum corneum ex vivo at the use of ethanol as a diffusion enhancer: (6.85±3.75)×10−7 cm2/s.

A Complex Approach for the Study of Biofilms of Microorganisms by Atomic Force Microscopy

The biofilms of Staphylococcus aureus and Escherichia coli have been investigated by atomic force microscopy (AFM). The usage of complex approach of semi-contact AFM mode has shown that the S. aureus and E. coli biofilms represent an organized community of microorganisms. Semicontact error mode and phase imaging mode investigation of extracellular matrix of S. aureus and E. coli biofilms, confirms dimensions of bacteria and biofilms. Semicontact mode let a development S-layer on surface of microorganisms, contact – different in adhesive force of bacteria. 

Application of Atomic Force Microscopy for Detection of Influence of Antibiotic Upon the Microbial Cell (on the Model of E.coli and I Generation Cephalosporins)

Alteration of E.coli cell wall caused by Cefazolin-AKOS was observed atomic force microscopy (AFM).Using semi-contact and contact modes the damaging effect of the Cefazolin-AKOS antibiotic was shown after a 30 minutes exposure. The assessment of physical parameters of cell allow to get more detailed information on the effect of antibiotics on microorganisms.

Rotational Isomeric and Vibrational Spectra of Monomers and Dimers for Acrylic Acid

In frame of method DFT/b3LYP modelling calculation of geometrical structure and vibrational spectra for monomers and dimmers of trans- and cis- isomers of acrylic acid are carried out. The influence of anharmonic resonance effects and intermolecular interection on state and intensities of bands in IR and Raman spectra are researched.