Izvestiya of Saratov University.

Physics

ISSN 1817-3020 (Print)
ISSN 2542-193X (Online)

For citation:

Bibikova O. A., Staroverov S. A., Sokolov O. I., Dikman L. A., Bogatyrev V. A. PlasmonResonance Gold Nanoparticles as Drug Carriers and Optical Labels for Cytological Investigations. Izvestiya of Saratov University. Physics , 2011, vol. 11, iss. 2, pp. 58-61. DOI: 10.18500/1817-3020-2011-11-2-58-61

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
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Language: 
Russian
Heading: 
Physics
UDC: 
576.3
DOI: 
10.18500/1817-3020-2011-11-2-58-61

PlasmonResonance Gold Nanoparticles as Drug Carriers and Optical Labels for Cytological Investigations

Autors: 
Bibikova Olga Aleksandrovna, Saratov State University
Staroverov Sergej Aleksandrovich, Saratov Veterinary Research Station RAAS
Sokolov Oleg Igorevich, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS)
Dikman Lev Abramovich, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS)
Bogatyrev Vladimir Aleksandrovich, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS)
Abstract: 

In this paper we report on the investigation of the influence of plasmon resonant gold nanoparticles (PRGNPs) and their complex with anticancer drug prospidin to the physiological functions (endocytosis, respiratory activity, viability) of tumor cell lines HeLa and SPEV2. We used the combination of several microscopic regimes to register the light scattering and fluorescence for visualization the cells and PRGNPs. The citratestabilized colloidal gold with average particle diameter 50 nm was used as plasmon resonant labels. The use of PRGNPs with subsequent staining by cationic fluorescent dye acridine orange enabled to visualize the labels by light microscopy and estimate their localization inside the cells by the confocal microscopy. We showed the enhancement of cytostatic activity of prospidin in the complex with the colloidal gold.

Key words: 
plasmon-resonance gold nanoparticles
fluorescent labels
confocal microscopy
differential interference contrast microscopy
dark-field microscopy
viability
endocytosis
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