Biophysics and medical physics

Leukocytes’ «Highlighting» Effect and its Application to Identify Blood Cells by Digital Microscopy Method

Objective: To find a way of identifying and counting of leukocytes in a native blood sample. Materials and equipments: Whole donor blood sample, digital microscop. Methods and approaches: The development of a method of leukocytes’ identification and counting for native blood samples was carried out on the basis of digital microscopy method. Main results: Leukocytes’ “highlighting” effect in a native blood sample was revealed experimentally by digital microscope examining.

Albumin Conformational Changes During Glycation and Thermal Denaturation Processes Revealed by Fluorescence Spectroscopy and Small-angle X-ray Scattering

Background and Objectives: Objects of the research in this study are solutions of bovine serum albumin (BSA) and its aggregates. Structural changes of the protein molecules in solution with pH 3 and pH 7.4 are investigated during glycation and thermal denaturation processes, when the BSA molecules in solution undergo similar intermediate states. The main aim of the research is to compare structural changes of the BSA upon its glycation and thermal denaturation, revealed by combination of optical and X-ray techniques.

Investigation of the Interaction and Dynamics of Collagen and Collagenase Molecules in Solutions by Dynamic Light Scattering

Background and Objectives: Bacterial collagenase from Closrtidium histolyticum is widely used as a clinical tool in the nonsurgical treatment of Dupuytren’s disease in eye’s disorders treatment, for enzymatic debridement, for accelerated resorption of catgut sutures. Collagenase main feature is its ability to digest key protein of the animal extracellular matrix – collagen. Dynamic Light Scattering (DLS) technique allows for investigation of collagen and collagenase solutions in conditions close to physiological.

Monte Carlo Simulation of Laser Radiation Propagation in the Multilayers Model of Head and Brain Tissues in Health and in the Presence of Intracranial Hematoma

Background and Objectives: Development of new optical methods of non-contact express diagnostics of intracranial hematoma remains an actual task. The development of optical model of the head in norm and in the presence of intracranial hematoma is the aim of the present study. Influence of the dimensions of the head tissues with and without hematoma on distribution of the backscattered laser radiation intensity is discussed. Materials and Methods: The optical model of the head and brain tissues in norm and in the presence of intracranial hematoma is developed.

Improved Data Processing Algorithm for Laser Ektacytometry of Red Blood Cells

Background and Objectives: Red blood cells deliver oxygen to organs and tissues. In case of tropical malaria, blood anemia, diabetes mellitus and many other diseases, the cells become corrupted. These pathologies lead in altering deformability of the cells, i.e ability to change their shape under external forces. Precise measurement of cell’s deformability gives important medical information which helps to cure and monitor the most wide spread diseases more effectively.

Optical Study of Blood Rheological Properties for Krushinsky – Molodkina Strain Rats with Diabetes Mellitus and Acute Disturbances of the Cerebral Circulation

Background and Objectives: The aim of the study was to assess the hemorheological parameters for rats with experimental diabetes and acute disturbances of the cerebral circulation. Materials and Methods: For modeling of the acute cerebral circulation disturbances (ACCD) the epilepsy-prone rats of Krushinsky–Molodkina strain were used. During the application of the specifically elaborated standard procedure of sound stimulation a strong sound impact leads to ACCD predominantly of the hemorrhagic type.

Optical Clearing of Cranial Bone by Multicomponent Immersion Solutions and Cerebral Venous Blood Flow Visualization

Background and Objectives: Optical clearing of bone tissue is of great practical interest, which opens up the possibility of the development of minimally invasive laser diagnostics and brain therapy. The aim of this work is the study of the optical clearing of cranial bone using multicomponent optical clearing agents, and the possibility of the measurement of cerebral blood flow.

Applying Methods of Diffuse Light Scattering and Optical Trapping for Assessing Blood Rheological Parameters: Erythrocytes Aggregation in Diabetes Mellitus

Background and Objectives: Aggregation parameters of blood characterize red blood cells interaction processes which play a major role in the microcirculation regulation. It was shown that these parameters are significantly different in case of Diabetes Mellitus (DM) and therefore can be proposed as a novel parameter of the disease state and therapy efficiency.

Investigation of Delay Time in Interaction between the Regulatory Circuits in the Cardiovascular System of Healthy Humans Using Modeling of Phase Dynamics

Background and Objectives: Low-frequency oscillations with a basic frequency of about 0.1 Hz are observed in the human heart rate and peripheral microcirculation. It is found out that these processes are self-oscillatory and interact between themselves. However, the details and characteristics of this interaction including the direction of coupling and delays in coupling functions are not well studied yet.

Influence of the Hydrogen Bond on the IR-spectrum and Structure of Molecular Complex of Diamond Nanoparticles and DNA Bases

Background and Objectives: Using molecular modeling by the density functional theory method we analyze a hydrogen bonds formation and their influence on IR-spectrum and structure of molecular complex which is formed as the interaction of complementary couple of DNA nucleobases adenine and timin and nanodiamonds surrounded with carboxylic groups. As an example of nanodiamonds adamantine has been used. Intermolecular forces and structure of hydrogen bonds are investigated.